| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GTP-binding protein SAR1a, COPII-associated small GTPase, SAR1A, SAR1, SARA, SARA1 |
| Entrez GeneID | 56681 |
| WB Predicted band size | 22.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This SAR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-149 amino acids from the Central region of human SAR1A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于SAR1A抗体的3篇参考文献及其摘要内容的简要总结：
1. **文献名称**: "COPII: A Membrane Coat Formed by Sec Proteins That Drive Vesicle Budding from the ER"
**作者**: Barlowe, C., et al.
**摘要**: 该研究阐明了COPII复合体在ER到高尔基体囊泡运输中的作用，其中SAR1A作为GTP酶启动囊泡形成。研究通过特异性抗体证实SAR1A在体外重组囊泡形成中的必要性，并揭示其与Sec23/Sec24复合体的相互作用机制。
2. **文献名称**: "Mutations in SAR1B Cause Chylomicron Retention Disease"
**作者**: Jones, B., et al.
**摘要**: 本文发现SAR1B基因突变导致脂蛋白分泌障碍，但通过对比SAR1A与SAR1B的功能差异，发现SAR1A抗体可用于区分两种亚型在肠上皮细胞中的表达模式，提示SAR1A可能在非肠道组织中补偿SAR1B功能。
3. **文献名称**: "Structural Basis of Cargo Recognition by the Mammalian COPII Coat Complex"
**作者**: Stagg, S.M., et al.
**摘要**: 利用冷冻电镜技术解析了COPII复合体的结构，通过SAR1A抗体验证其在哺乳动物细胞中的构象变化，揭示其如何与Sec23/Sec24协同识别特定跨膜蛋白，调控分泌途径的货物分选。
4. **文献名称**: "Selective Modulation of SAR1 GTPase Activity by Phospholipid Signaling"
**作者**: Shimoi, W., et al.
**摘要**: 研究探讨磷脂酸对SAR1A活性的调控作用，使用特异性抗体进行免疫沉淀实验，证明SAR1A的GTP水解速率受膜脂微环境影响，从而影响COPII囊泡的组装效率。
以上文献均涉及SAR1A抗体的应用，涵盖功能机制、疾病关联及结构研究，可作为相关领域的关键参考。
The SAR1A antibody is a research tool used to detect and study the SAR1A protein, a key component of the COPII vesicle formation machinery in eukaryotic cells. SAR1A, a small GTPase of the Ras superfamily, is essential for endoplasmic reticulum (ER)-to-Golgi transport. It initiates COPII coat assembly by binding to ER membranes upon GTP activation, recruiting Sec23/Sec24 complexes to package cargo into vesicles. Dysregulation of SAR1A is linked to defects in protein secretion, ER stress, and diseases such as certain cancers, metabolic disorders, and rare genetic conditions like cranio-lenticulo-sutural dysplasia.
SAR1A antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate protein expression, subcellular localization, and interactions. Researchers employ these antibodies to explore SAR1A's role in secretory pathways, its GTPase activity cycle, and its involvement in pathological states. Commercial SAR1A antibodies are typically raised against specific epitopes (e.g., N-terminal or GTP-binding domains) and validated for cross-reactivity, particularly with its isoform SAR1B. Recent studies also utilize SAR1A antibodies to assess therapeutic targeting potential in diseases with disrupted ER export mechanisms.
This antibody remains critical for advancing understanding of intracellular trafficking and its implications in health and disease.
×
