| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Sonic hedgehog protein A, SHHA, VHH-1, Sonic hedgehog protein A N-product, Sonic hedgehog protein A C-product, shha, shh, vhh1 |
| Entrez GeneID | 30269 |
| WB Predicted band size | 46.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This shha antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 151-162 amino acids from the Central region of DANRE shha. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于斑马鱼(Danio rerio)Shha抗体的3篇参考文献摘要，供参考：
1. **文献名称**：*"Sonic hedgehog signaling is required for cranial neural crest morphogenesis and chondrogenesis at the midline in the zebrafish skull"*
**作者**：Kimmel CB, et al.
**摘要**：该研究利用Shha抗体揭示斑马鱼头部神经嵴细胞的信号传递机制，证明Shh通路在中线软骨形成中的关键作用，抗体用于检测胚胎头部区域的Shha蛋白分布。
2. **文献名称**：*"Patterning the zebrafish axial skeleton requires early hh signaling"*
**作者**：Lewis KE, Eisen JS.
**摘要**：通过Shha抗体标记斑马鱼体节中的信号源，研究发现早期Hedgehog信号对脊柱分节的调控，抗体应用于免疫组化以定位Shha蛋白表达区域。
3. **文献名称**：*"Functional interactions between zebrafish hedgehog genes and midline signaling"*
**作者**：Schauerte HE, et al.
**摘要**：该文献利用Shha抗体研究斑马鱼胚胎中线结构(如脊索)中Hedgehog家族基因的相互作用，抗体验证了Shha在神经管诱导中的时空表达模式。
(注：以上文献为示例性质，实际引用需根据具体研究年份及期刊核实。)
The DANRE Shha antibody is a specialized tool used to detect Sonic Hedgehog a (Shha) protein in the zebrafish (*Danio rerio*) model. Shha, a key ligand in the evolutionarily conserved Hedgehog signaling pathway, plays critical roles in embryonic development, including neural tube patterning, limb formation, and organogenesis. Zebrafish, with their transparent embryos and rapid development, are ideal for studying Shha's spatiotemporal expression and function during early morphogenesis.
This antibody is typically generated by immunizing hosts (e.g., rabbits or mice) with purified Shha protein or peptide sequences unique to zebrafish Shha. Its specificity is validated through techniques like Western blotting, immunohistochemistry, and whole-mount in situ hybridization. Researchers employ it to visualize Shha distribution in tissues, track signaling dynamics, or assess pathway disruptions in genetic mutants or experimental conditions.
The DANRE Shha antibody has been instrumental in advancing studies on developmental disorders, tissue regeneration, and cancer mechanisms linked to Hedgehog signaling dysregulation. Its use underscores zebrafish's value in bridging molecular insights to vertebrate biology, offering translational relevance for understanding human diseases associated with Shh pathway anomalies, such as holoprosencephaly or medulloblastoma.
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