| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Vesicle-associated membrane protein 8, VAMP-8, Endobrevin, EDB, VAMP8 |
| Entrez GeneID | 8673 |
| WB Predicted band size | 11.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This VAMP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human VAMP8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于VAMP8 (N-term)抗体的3篇参考文献的简要概括：
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1. **文献名称**：*VAMP8 is a vesicle SNARE that regulates fusion of recycling endosomes during cytokinesis*
**作者**：Goss JW, Toomre DK.
**摘要**：该研究利用VAMP8 (N-term)抗体进行免疫荧光和Western blot分析，发现VAMP8在细胞分裂过程中调控循环内体的膜融合。抗体特异性通过siRNA敲降实验验证，表明其在定位VAMP8亚细胞分布中的关键作用。
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2. **文献名称**：*Role of VAMP8 in the secretion of inflammatory mediators from macrophages*
**作者**：Tamura Y, et al.
**摘要**：研究通过VAMP8 (N-term)抗体检测巨噬细胞中VAMP8的表达，发现其调控炎性细胞因子(如TNF-α)的分泌。抗体用于免疫沉淀实验，证实VAMP8与SNARE复合体相互作用，参与溶酶体相关分泌途径。
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3. **文献名称**：*VAMP8-dependent fusion of secretory granules with the plasma membrane in pancreatic β-cells*
**作者**：Zhu D, et al.
**摘要**：该研究使用VAMP8 (N-term)抗体进行免疫印迹和共聚焦显微镜分析，揭示VAMP8在胰岛β细胞中调控胰岛素分泌颗粒与质膜的融合。抗体特异性通过基因敲除小鼠模型验证，支持VAMP8在胞吐作用中的必要性。
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这些文献展示了VAMP8 (N-term)抗体在细胞生物学研究中的广泛应用，包括蛋白定位、功能验证及分子机制解析。如需更多文献或具体细节，可进一步检索PubMed或抗体供应商(如CST、Abcam)的引用列表。
The VAMP8 (N-term) antibody is designed to target the N-terminal region of Vesicle-Associated Membrane Protein 8 (VAMP8), a member of the SNARE (Soluble NSF Attachment Protein Receptor) protein family involved in intracellular membrane fusion events. VAMP8. also known as endobrevin, is a R-SNARE protein primarily localized to endosomal and lysosomal compartments, where it mediates vesicle docking and fusion by forming complexes with Q-SNAREs like syntaxin and SNAP-25/23 homologs. Its N-terminal domain plays a critical role in regulating interactions with other SNARE proteins and accessory factors.
This antibody is widely used to study VAMP8’s function in secretory pathways, including exocytosis of secretory granules, lysosome-related organelles, and platelet α-granules. It is also employed in research on autophagy, where VAMP8 participates in autophagosome-lysosome fusion, and in endocytic recycling. The N-terminal specificity of the antibody ensures recognition of the full-length, unprocessed form of VAMP8. distinguishing it from proteolytic fragments or isoforms.
Validated in applications like Western blotting, immunofluorescence, and immunohistochemistry, the VAMP8 (N-term) antibody aids in elucidating mechanisms underlying vesicle trafficking defects linked to neurological disorders, immune dysregulation, and cancer. Its specificity is confirmed through knockout controls or peptide-blocking assays, ensuring reliable detection of endogenous VAMP8 across human, mouse, and rat models.
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