| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Acyl-CoA synthetase family member 2, mitochondrial, 621-, ACSF2 |
| Entrez GeneID | 80221 |
| WB Predicted band size | 68.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ACSF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 587-615 amino acids from the C-terminal region of human ACSF2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ACSF2抗体的3篇示例参考文献(内容为模拟概括，具体文献需通过学术数据库核实)：
1. **"Characterization of ACSF2-specific antibodies for mitochondrial acyl-CoA synthetase studies"**
- **作者**: Smith J, et al. (2016)
- **摘要**: 本研究开发并验证了一种针对人源ACSF2蛋白的多克隆抗体，通过Western blot和免疫荧光证实其特异性。抗体成功用于检测ACSF2在线粒体中的定位，并揭示其在脂肪酸氧化通路中的功能缺失表型。
2. **"ACSF2 expression profiling in cancer using a novel monoclonal antibody"**
- **作者**: Tanaka R, et al. (2019)
- **摘要**: 报道了一种高灵敏度小鼠单克隆抗体的开发，用于免疫组化分析ACSF2在多种肿瘤组织中的表达差异。研究发现ACSF2低表达与乳腺癌患者预后不良相关，提示其潜在临床意义。
3. **"Validation of ACSF2 antibody for functional studies in neuronal metabolism"**
- **作者**: Lee S, et al. (2021)
- **摘要**: 通过CRISPR/Cas9敲除细胞系验证了商业ACSF2抗体(货号#AB123. XYZ公司)的特异性，并应用于脑组织切片染色，发现ACSF2在小鼠海体神经元中的高表达，支持其在神经脂质代谢中的作用。
**建议**：实际文献需通过PubMed、Web of Science等平台以“ACSF2 antibody”、“ACSF2 validation”等关键词检索，部分抗体厂商(如Abcam、CST)的产品说明书也可能引用相关文献。
The ACSF2 (acyl-CoA synthetase family member 2) antibody is a tool used to study the function and expression of the ACSF2 protein, an enzyme involved in fatty acid metabolism. ACSF2 belongs to the acyl-CoA synthetase family, which activates fatty acids by conjugating them with coenzyme A (CoA) to form acyl-CoA derivatives, a critical step in lipid biosynthesis and energy production. Specifically, ACSF2 is localized to mitochondria and plays a role in mitochondrial fatty acid activation, influencing metabolic pathways such as β-oxidation.
Research using ACSF2 antibodies has focused on understanding its expression patterns in tissues like liver, heart, and skeletal muscle, where fatty acid metabolism is highly active. Studies suggest ACSF2 dysregulation may link to metabolic disorders, cancer, and neurodegenerative diseases. The antibody is commonly utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein levels, subcellular localization, and tissue distribution.
Recent investigations highlight ACSF2's potential role in maintaining mitochondrial function and cellular energy homeostasis, making it a target for metabolic syndrome or aging-related research. Commercially available ACSF2 antibodies are typically validated for specificity in human, mouse, or rat samples, aiding cross-species studies. However, variability in post-translational modifications or isoform expression requires careful experimental optimization. Ongoing work aims to clarify its regulatory mechanisms and therapeutic relevance in metabolic diseases.
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