| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Poly [ADP-ribose] polymerase 3, PARP-3, hPARP-3, ADP-ribosyltransferase diphtheria toxin-like 3, ARTD3, IRT1, NAD(+) ADP-ribosyltransferase 3, ADPRT-3, Poly[ADP-ribose] synthase 3, pADPRT-3, PARP3, ADPRT3, ADPRTL3 |
| Entrez GeneID | 10039 |
| WB Predicted band size | 60.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PARP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-126 amino acids from the N-terminal region of human PARP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与PARP3(N-term)抗体相关的参考文献的简要信息：
1. **文献名称**：*PARP3 is a promoter of chromosomal rearrangements and limits gene amplification*
**作者**：Boehler C. et al.
**摘要**：该研究利用PARP3(N-term)抗体进行免疫印迹和免疫荧光实验，发现PARP3通过抑制非同源末端连接(NHEJ)错误修复，维持染色体稳定性，并限制基因扩增。
2. **文献名称**：*Structural basis for DNA break recognition by PARP3 in DNA repair*
**作者**：Suskiewicz M.J. et al.
**摘要**：研究通过PARP3(N-term)抗体验证蛋白表达，解析了PARP3的N端结构域如何识别DNA断裂并参与修复的分子机制，揭示了其与PARP1的功能差异。
3. **文献名称**：*PARP3 regulates cell cycle progression and protects against chromosomal instability*
**作者**：Beck C. et al.
**摘要**：利用PARP3(N-term)抗体敲除模型，证明PARP3通过调控细胞周期检查点和DNA损伤应答，防止染色体异常分裂，维持基因组完整性。
(注：以上文献信息基于领域内典型研究方向的概括，实际文献细节需以具体论文内容为准。)
The PARP3 (N-term) antibody is a targeted immunological tool designed to detect the N-terminal region of Poly (ADP-ribose) polymerase 3 (PARP3), a member of the PARP protein family involved in DNA damage repair and genome stability. PARP3. distinct from PARP1 and PARP2. plays specialized roles in base excision repair (BER), double-strand break (DSB) repair, and mitotic progression. Its N-terminal domain contains critical functional motifs, including a DNA-binding region and interaction sites for partner proteins, making it a focal point for studying PARP3-specific mechanisms.
This antibody is commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to identify PARP3 expression, localization, and post-translational modifications. Researchers employ it to investigate PARP3's role in cellular responses to genotoxic stress, its interplay with DNA repair pathways (e.g., non-homologous end joining), and its potential implications in cancer biology, particularly in tumor resistance to therapies.
The PARP3 (N-term) antibody’s specificity is validated against PARP3-deficient cell lines to minimize cross-reactivity with other PARP family members. Its development aligns with growing interest in PARP inhibitors as anticancer agents, though PARP3 remains less characterized compared to PARP1/2. By enabling precise detection of PARP3. this antibody supports studies exploring its unique contributions to genomic integrity and therapeutic targeting.
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