Rabbit Polyclonal Phospho-ratTSC1(S312) Antibody

The Phospho-rat TSC1(S312) antibody is a specialized tool for detecting the phosphorylation status of threonine 312 (T312) in the tuberous sclerosis complex 1 (TSC1) protein in rat-derived samples. TSC1. in complex with TSC2. acts as a critical regulator of the mTOR (mechanistic target of rapamycin) signaling pathway, which controls cell growth, proliferation, and metabolism. Phosphorylation of TSC1 at specific residues, including S312 (or T312 in some species), modulates its ability to inhibit mTORC1 activity, thereby influencing downstream processes like protein synthesis and autophagy. This phosphorylation event is often linked to cellular responses to growth factors, energy status, or stress signals. The Phospho-rat TSC1(S312) antibody is commonly used in Western blotting, immunohistochemistry, or immunofluorescence to study mTOR pathway regulation in rat models of cancer, metabolic disorders, or neurological diseases. Its specificity for the phosphorylated form of TSC1 allows researchers to assess pathway activation under experimental conditions, such as drug treatments or genetic manipulations. Validations typically include testing in knockout models or phosphatase-treated lysates to confirm target specificity. This antibody supports research exploring mTOR-related diseases, including tuberous sclerosis complex, tumors, and insulin resistance.