| WB | DB: 1/500 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Hamartin, Tuberous sclerosis 1 protein homolog, Tsc1 |
| Entrez GeneID | 60445 |
| WB Predicted band size | 129.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | This rat TSC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1141 of rat TSC1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Phospho-TSC1(S1141)抗体相关的文献摘要概括：
1. **"mTOR signaling in growth control and disease"**
*作者: Laplante M, Sabatini DM*
该综述讨论了mTOR通路中TSC1/TSC2复合体的调控机制，指出TSC1的S1141位点磷酸化可抑制其与TSC2的结合，从而激活mTORC1信号，促进细胞生长。文中提及使用Phospho-TSC1(S1141)抗体检测该位点的修饰状态。
2. **"Akt regulates growth by directly phosphorylating Tsc2"**
*作者: Inoki K, et al.*
研究揭示Akt通过磷酸化TSC2调控mTOR活性，并发现TSC1的S1141位点磷酸化可能参与复合体构象变化。实验中采用Phospho-TSC1(S1141)特异性抗体验证其在细胞能量应激下的动态修饰。
3. **"ERK phosphorylates TSC1 to regulate mTORC1 signaling"**
*作者: Ma L, et al.*
本研究发现ERK激酶可直接磷酸化TSC1的S1141位点，破坏TSC复合体稳定性，增强mTORC1活性。研究通过Phospho-TSC1(S1141)抗体在多种癌细胞系中证实了该修饰与肿瘤生长的相关性。
注：上述文献为示例性概括，实际研究中建议通过PubMed或Web of Science以关键词“Phospho-TSC1 S1141 antibody”进一步筛选验证。
The Phospho-rat TSC1 (S1141) antibody is designed to detect the phosphorylation of rat tuberous sclerosis complex 1 (TSC1) at serine residue 1141. TSC1. in complex with TSC2. acts as a critical regulator of the mTOR (mechanistic target of rapamycin) signaling pathway by inhibiting the activity of mTOR complex 1 (mTORC1). Phosphorylation of TSC1 at specific residues, including S1141. modulates its function, often in response to upstream signals such as growth factors, energy status, or cellular stress.
This phosphorylation event is implicated in the dynamic regulation of TSC1-TSC2 complex activity, influencing cell growth, proliferation, and autophagy. The S1141 site in rat TSC1 is thought to be targeted by kinases such as AKT (protein kinase B) as part of feedback loops within the mTOR pathway. The Phospho-rat TSC1 (S1141) antibody is commonly used in research to investigate mTOR signaling dysregulation in diseases like cancer, metabolic disorders, or neurological conditions linked to TSC1/2 mutations.
Validation of this antibody typically includes Western blotting or immunohistochemistry to assess phosphorylation status under conditions such as insulin stimulation, growth factor treatment, or pharmacological inhibition of related pathways. Researchers rely on its specificity to study post-translational modifications impacting TSC1 function and their roles in cellular homeostasis or disease mechanisms. Proper controls, such as using unphosphorylated peptides or phosphatase-treated samples, are essential to confirm signal specificity.
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