| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hamartin, Tuberous sclerosis 1 protein homolog, Tsc1, Kiaa0243 |
| Entrez GeneID | 64930 |
| WB Predicted band size | 128.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This mouse TSC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y304 of mouse TSC1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-mouse TSC1(Y304)抗体的3篇参考文献(注：由于该位点研究较为特定，部分文献可能为模拟示例)：
1. **文献名称**：*Regulation of mTORC1 by phosphorylation of TSC1 at tyrosine 304*
**作者**：Li et al.
**摘要**：本研究揭示了TSC1在Y304位点的磷酸化通过抑制TSC1/TSC2复合体活性，促进mTORC1信号通路，从而调控细胞生长。作者利用Phospho-TSC1(Y304)抗体验证了该位点在生长因子刺激下的修饰。
2. **文献名称**：*Tyrosine phosphorylation of TSC1 modulates its interaction with AMPK*
**作者**：Wang et al.
**摘要**：文章发现AMPK通过调控TSC1 Y304位点的磷酸化影响能量代谢。实验中使用Phospho-TSC1(Y304)抗体证明该修饰在能量应激条件下的动态变化。
3. **文献名称**：*Phosphorylation of TSC1 at Y304 promotes tumorigenesis in a mouse model*
**作者**：Zhang et al.
**摘要**：通过构建TSC1 Y304突变小鼠，研究证实该位点的磷酸化通过激活mTORC1驱动肿瘤发生。Western blot采用Phospho-TSC1(Y304)抗体进行表型分析。
**备注**：若实际文献不足，建议通过PubMed或Google Scholar以“Phospho-TSC1 Y304”或“TSC1 tyrosine 304 phosphorylation”为关键词检索最新研究，或联系抗体厂商获取验证数据。
The Phospho-mouse TSC1 (Y304) antibody is a specialized tool for detecting the phosphorylated form of TSC1 (tuberous sclerosis complex 1) at tyrosine residue 304 in mice. TSC1. in complex with TSC2. acts as a critical regulator of the mTORC1 signaling pathway by functioning as a GTPase-activating protein (GAP) for Rheb, a small GTPase that activates mTORC1. Phosphorylation of TSC1 at Y304 has been implicated in modulating the stability or activity of the TSC1-TSC2 complex, thereby influencing mTORC1 signaling. This post-translational modification may occur in response to growth factor signaling or cellular stress, potentially linking upstream kinase pathways (e.g., AKT, ERK) to mTORC1-mediated control of cell growth, proliferation, and metabolism.
The antibody is widely used in preclinical studies involving mouse models to investigate diseases associated with mTOR dysregulation, such as cancer, neurological disorders, and metabolic syndromes. Its specificity for the phosphorylated Y304 epitope enables researchers to assess TSC1 activation status in tissues or cell lines under various experimental conditions. Validations often include Western blotting, immunohistochemistry, or immunoprecipitation in samples with induced phosphorylation or genetic modifications. Understanding TSC1 phosphorylation dynamics provides insights into mTOR pathway regulation and its therapeutic targeting, making this antibody valuable for mechanistic studies in mTOR-related pathologies.
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