Rabbit Polyclonal Phospho-mouseCCNB3(T258) Antibody

Phospho-mouse CCNB3 (Thr258) antibody is a specialized tool designed to detect the phosphorylated form of cyclin B3 (CCNB3) at threonine residue 258 in mouse-derived samples. CCNB3. a member of the B-type cyclin family, plays a critical role in regulating the cell cycle, particularly during the transition from G2 to M phase and in meiosis. Phosphorylation at Thr258 is believed to modulate CCNB3’s interaction with cyclin-dependent kinases (CDKs) or its subcellular localization, influencing its function in cell cycle progression and checkpoint control. This antibody is generated by immunizing hosts with a synthetic phosphopeptide corresponding to the Thr258 region, ensuring specificity for the phosphorylated epitope. It is commonly used in techniques like Western blotting, immunohistochemistry, or immunofluorescence to study cell cycle dynamics, meiotic regulation, or DNA damage responses in mouse models. Researchers also utilize it to explore pathological conditions linked to cell cycle dysregulation, such as cancer or reproductive disorders. Validation typically includes testing in knockout or phosphatase-treated samples to confirm phosphorylation-dependent reactivity. Proper storage conditions (e.g., -20°C in stabilizing buffers) are essential to maintain antibody activity. Its application contributes to understanding how post-translational modifications of CCNB3 impact cellular proliferation and genomic stability.