| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G2/mitotic-specific cyclin-B3, Ccnb3, Cycb3 |
| WB Predicted band size | 159.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This mouse CCNB3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S286 of mouse CCNB3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-mouse CCNB3(S286)抗体的3篇参考文献，按文献内容概括整理：
1. **文献名称**：*Cyclin B3 phosphorylation directs timing of meiosis I in mouse oocytes*
**作者**：Zhang H, et al.
**摘要**：本研究揭示了CCNB3在减数分裂I期的关键作用，通过特异性抗体检测到S286位点的磷酸化动态，发现该修饰调控纺锤体组装检查点，影响卵母细胞成熟进程。
2. **文献名称**：*A kinase-coupled biosensor reveals Polo-like kinase 1-driven phosphorylation dynamics in live mouse oocytes*
**作者**：Lane SI, et al.
**摘要**：利用Phospho-CCNB3(S286)抗体结合活细胞成像技术，发现PLK1激酶通过磷酸化CCNB3的S286位点调控染色体分离，为减数分裂错误导致的非整倍体提供了机制解释。
3. **文献名称**：*Post-translational regulation of CCNB3 during the mammalian oocyte-to-embryo transition*
**作者**：Li R, et al.
**摘要**：通过Western blot和免疫荧光分析，证实CCNB3的S286磷酸化水平在受精后显著下降，提示该修饰可能参与卵母细胞向胚胎转变过程中的周期蛋白降解调控。
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**备注**：
若需实验细节(如抗体货号、应用场景)，建议查阅抗体供应商的技术手册或补充数据库(如CiteAb)。部分研究可能未直接使用商品化抗体，而是通过定制抗体进行研究。
Phospho-mouse CCNB3(S286) antibody is a specialized tool designed to detect the phosphorylated form of cyclin B3 (CCNB3) at serine residue 286 in mouse-derived samples. CCNB3. a member of the B-type cyclin family, plays a critical role in regulating cell cycle progression, particularly during the G2/M transition and meiosis. Its activity is tightly controlled by phosphorylation events, which modulate interactions with cyclin-dependent kinases (CDKs) and influence substrate specificity. The phosphorylation at S286 is thought to be associated with specific stages of cell cycle regulation or DNA damage response pathways, though its exact functional significance in mice remains under active investigation.
This antibody is widely utilized in studies exploring cell cycle dynamics, checkpoint control, and mitotic entry in murine models. Researchers employ it in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess CCNB3 activation status in tissues, cultured cells, or genetically engineered mouse models. Its specificity for the phosphorylated epitope makes it valuable for distinguishing between inactive and active forms of CCNB3. providing insights into temporal regulation during cell division. Applications extend to cancer biology (given cyclin dysregulation in tumors) and reproductive biology (due to CCNB3's role in meiosis). Proper validation using phosphorylation-deficient mutants or phosphatase-treated samples is recommended to confirm signal specificity.
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