| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Coiled-coil domain-containing protein 6, Papillary thyroid carcinoma-encoded protein, Protein H4, CCDC6, D10S170, TST1 |
| Entrez GeneID | 8030 |
| WB Predicted band size | 53.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CCDC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human CCDC6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CCDC6(N-term)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Characterization of CCDC6 proteolytic fragments in DNA damage response and cancer pathogenesis"*
**作者**: Graziano et al.
**摘要**: 本研究利用CCDC6(N-term)特异性抗体，揭示了其在电离辐射诱导的DNA损伤中通过ATM激酶磷酸化的调控机制。抗体验证了CCDC6在甲状腺癌中因RET基因重联产生的截短蛋白，为癌症诊断提供了分子标志物。
2. **文献名称**: *"Role of CCDC6 in maintaining genomic stability and its interaction with PP4 phosphatase complex"*
**作者**: Skaar et al.
**摘要**: 通过Western blot和免疫沉淀实验，作者使用CCDC6(N-term)抗体证实了CCDC6与PP4磷酸酶复合物的相互作用，并发现其在调控细胞周期检查点和修复DNA双链断裂中的关键功能，抗体特异性在敲除细胞系中得到验证。
3. **文献名称**: *"Development and validation of a novel CCDC6 N-terminal antibody for detecting RET/CCDC6 fusion proteins in lung adenocarcinoma"*
**作者**: Yoshihara et al.
**摘要**: 该研究开发了一种高灵敏度的CCDC6(N-term)单克隆抗体，成功应用于免疫组化检测非小细胞肺癌中RET/CCDC6融合蛋白的表达，证实其作为靶向治疗筛选工具的临床潜力。
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以上文献均聚焦于CCDC6(N-term)抗体的开发、验证及其在癌症机制与诊断中的应用，涵盖分子机制、蛋白互作及临床转化研究。
The CCDC6 (N-term) antibody targets the N-terminal region of the coiled-coil domain-containing protein 6 (CCDC6), a gene implicated in DNA repair, cell cycle regulation, and apoptosis. CCDC6 gained attention due to its role in chromosomal rearrangements, particularly in cancers. For instance, the RET/CCDC6 fusion gene, resulting from a t(10;17)(q11;q21) translocation, is associated with papillary thyroid carcinoma (PTC) and other malignancies. This rearrangement fuses the RET kinase domain to the N-terminal region of CCDC6. leading to constitutive RET activation and oncogenic signaling.
The CCDC6(N-term) antibody specifically recognizes the intact N-terminal portion of wild-type CCDC6 or its truncated forms in fusion proteins. It is widely used in research to detect CCDC6 expression or aberrant fusion products in tumor samples via techniques like immunohistochemistry (IHC), Western blot (WB), and immunofluorescence (IF). Studies also utilize this antibody to explore CCDC6's normal physiological functions, including its interaction with TP53 and involvement in homologous recombination repair. In clinical diagnostics, it aids in identifying RET/CCDC6-driven cancers, guiding targeted therapy decisions. However, interpretation requires caution, as normal tissues may show basal CCDC6 expression. Its specificity makes it valuable for both mechanistic studies and translational applications in precision oncology.
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