| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | 86.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S670 of human IKKB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及Phospho-IKKβ(S670)抗体的参考文献示例(内容为模拟，仅供参考)：
1. **"IKKβ phosphorylation at Ser670 controls NF-κB activation in response to oxidative stress"**
- **作者**: Li et al., 2016
- **摘要**: 研究发现IKKβ在S670位点的磷酸化是氧化应激诱导NF-κB通路激活的关键步骤，通过该抗体验证了此位点与细胞抗氧化能力的关系。
2. **"A novel phosphorylation site on IKKβ regulates Toll-like receptor signaling"**
- **作者**: Müller et al., 2018
- **摘要**: 利用Phospho-IKKβ(S670)抗体，揭示了TLR4信号通路中S670磷酸化对IKK复合物构象的影响，及其在炎症反应中的调控作用。
3. **"Targeting IKKβ Ser670 phosphorylation inhibits tumor growth in colorectal cancer models"**
- **作者**: Chen & Wang, 2020
- **摘要**: 通过该抗体证实S670磷酸化与结直肠癌中IKKβ的持续激活相关，靶向此位点可抑制肿瘤生长并增强化疗敏感性。
4. **"Antibody-specific detection of IKKβ phospho-Ser670 in autoimmune disease models"**
- **作者**: Gupta et al., 2019
- **摘要**: 开发并验证了Phospho-IKKβ(S670)抗体的特异性，应用于类风湿性关节炎模型，证明该位点磷酸化水平与疾病严重程度正相关。
**注**：以上为模拟文献，实际引用需查询PubMed或学术数据库(关键词：IKKβ Ser670 phosphorylation antibody)。若研究较少，建议确认位点编号准确性(如是否为Ser677或Ser181等常见位点)。
The Phospho-IKKβ (S670) antibody is a crucial tool for studying the activation dynamics of the inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ), a key regulator of the NF-κB signaling pathway. IKKβ, a serine/threonine kinase, forms part of the IKK complex (IKKα/IKKβ/IKKγ) that phosphorylates IκB proteins, leading to their degradation and subsequent activation of NF-κB transcription factors. Phosphorylation at Serine 670 (S670) in IKKβ is associated with its activation, often triggered by pro-inflammatory cytokines (e.g., TNF-α, IL-1β), microbial components, or cellular stress. This post-translational modification enhances IKKβ kinase activity, facilitating downstream signaling involved in immune responses, apoptosis regulation, and cell survival.
The Phospho-IKKβ(S670) antibody specifically detects this phosphorylation event, enabling researchers to monitor IKKβ activation status in experimental models. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate NF-κB pathway activation in contexts such as inflammation, cancer, and autoimmune diseases. Dysregulation of IKKβ phosphorylation is implicated in chronic inflammatory disorders (e.g., rheumatoid arthritis) and tumorigenesis, making this antibody valuable for both mechanistic studies and therapeutic target validation. Proper controls, including total IKKβ and phosphorylation-deficient mutants, are essential to ensure specificity in experimental setups.
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