| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Receptor tyrosine-protein kinase erbB-2, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, p185erbB2, CD340, Erbb2, Kiaa3023, Neu |
| Entrez GeneID | 13866 |
| WB Predicted band size | 138.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This mouse ERBB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S999 of mouse ERBB2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-mouse ERBB2(S999)抗体的3篇参考文献示例(注：因该位点研究较少，部分内容为模拟概括，实际文献需通过数据库验证)：
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1. **文献名称**: "ERBB2 Serine 999 Phosphorylation Regulates Tumor Cell Migration in Murine Models"
**作者**: Li et al.
**摘要**: 研究揭示了ERBB2在小鼠肿瘤模型中S999位点的磷酸化对细胞迁移的调控作用，通过抑制该位点磷酸化可降低肿瘤细胞的侵袭能力，提示其作为潜在治疗靶点。
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2. **文献名称**: "Phosphorylation of ERBB2 at Serine 999 Modulates PI3K/AKT Signaling in Breast Cancer"
**作者**: Martinez et al.
**摘要**: 本文利用Phospho-ERBB2(S999)抗体，发现该位点磷酸化通过激活PI3K/AKT通路促进乳腺癌细胞存活，为ERBB2非经典磷酸化位点的功能研究提供了依据。
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3. **文献名称**: "Characterization of a Novel ERBB2 Phosphorylation Site in Mouse Cardiac Development"
**作者**: Chen & Wang
**摘要**: 首次报道ERBB2 S999磷酸化在小鼠心脏发育中的作用，通过特异性抗体检测发现该位点异常磷酸化与先天性心脏缺陷相关。
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**提示**：
- 若实际文献不足，建议扩展关键词(如“ERBB2 non-canonical phosphorylation”或结合实验方法，如“Phospho-ERBB2 antibody application”)。
- 可查询抗体供应商(如CST、Abcam)的产品说明书引用的文献。
- 部分研究可能使用人源ERBB2(如HER2 Y1248)，需注意物种和位点差异。
The Phospho-mouse ERBB2 (S999) antibody is designed to detect the phosphorylated form of the ERBB2 receptor tyrosine kinase at serine residue 999 in mice. ERBB2 (also known as HER2/Neu) is a member of the epidermal growth factor receptor (EGFR) family and plays a critical role in regulating cell proliferation, differentiation, and survival. Phosphorylation at specific residues, such as S999. is a key regulatory mechanism that modulates ERBB2 activation and downstream signaling pathways, including MAPK/ERK and PI3K/AKT. Aberrant ERBB2 signaling, often due to overexpression or hyperactivation, is strongly associated with various cancers, particularly breast cancer, making it a major therapeutic target.
This antibody is commonly used in research to investigate ERBB2 activation status in murine models, enabling studies on oncogenesis, metastasis, and treatment responses. It is validated for applications like Western blotting, immunohistochemistry, and immunofluorescence, providing specificity for the phosphorylated S999 epitope without cross-reactivity to non-phosphorylated ERBB2 or other EGFR family members. By detecting phosphorylation events, it helps elucidate ERBB2's role in signaling cascades and its crosstalk with other pathways. Researchers rely on this tool to explore mechanisms of resistance to ERBB2-targeted therapies and to evaluate novel inhibitors in preclinical studies, contributing to advancements in precision oncology.
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