| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Leucine-rich repeat-containing protein 42, LRRC42 |
| Entrez GeneID | 115353 |
| WB Predicted band size | 48.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LRRC42 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-71 amino acids from the Central region of human LRRC42. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LRRC42抗体的3-4篇参考文献的示例(注：以下内容为模拟示例，实际文献可能需要根据具体数据库检索验证)：
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1. **文献名称**: *LRRC42 regulates centrosome dynamics and mitotic progression via Aurora A kinase interaction*
**作者**: Chen L, et al.
**摘要**: 本研究利用特异性抗LRRC42抗体进行免疫沉淀和免疫荧光实验，发现LRRC42通过与Aurora A激酶结合调控中心体稳定性，影响细胞有丝分裂进程。抗体验证显示LRRC42在多种癌细胞系中高表达。
2. **文献名称**: *Development and validation of a polyclonal antibody against human LRRC42 for functional studies*
**作者**: Wang X, et al.
**摘要**: 文章报道了一种新型多克隆抗LRRC42抗体的开发，并通过Western blot和免疫组化验证其特异性。该抗体成功应用于检测LRRC42在小鼠脑组织中的亚细胞定位。
3. **文献名称**: *LRRC42 modulates ciliary length and Hedgehog signaling in vertebrate development*
**作者**: Gupta S, et al.
**摘要**: 通过抗LRRC42抗体的免疫染色，研究发现LRRC42缺失导致纤毛缩短并影响Hedgehog信号通路。抗体在斑马鱼模型中的应用证实了LRRC42在胚胎发育中的关键作用。
4. **文献名称**: *LRRC42 expression correlates with poor prognosis in gastric cancer patients*
**作者**: Tanaka K, et al.
**摘要**: 使用抗LRRC42抗体对胃癌组织进行免疫组化分析，发现LRRC42高表达与肿瘤侵袭性和患者生存率下降显著相关，提示其作为潜在生物标志物的价值。
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**说明**：若需真实文献，建议通过PubMed或Google Scholar以关键词“LRRC42 antibody”或“LRRC42 immunohistochemistry”检索近年研究，并筛选涉及抗体应用的实验(如WB、IF、IHC等)。部分研究可能侧重LRRC42功能而非抗体开发，但会包含抗体验证环节。
The LRRC42 antibody is a research tool targeting the leucine-rich repeat-containing protein 42 (LRRC42), a member of the leucine-rich repeat (LRR) superfamily involved in protein-protein interactions and cellular signaling. LRRC42 is implicated in regulating ciliary assembly, cell cycle progression, and microtubule dynamics. It interacts with components of the Hippo signaling pathway and may influence organ size control and tumor suppression. Studies suggest LRRC42's role in maintaining primary cilia structure, linking it to ciliopathies and cancer pathologies.
Antibodies against LRRC42 are primarily used to study its expression, localization, and molecular interactions in tissues or cultured cells. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercial LRRC42 antibodies are typically raised in rabbits or mice, with validation in specific applications. Researchers utilize these antibodies to explore LRRC42's involvement in cellular processes, such as its interaction with P53 or its dysregulation in tumors. However, antibody specificity remains a consideration, requiring validation using knockout controls. Current literature highlights LRRC42's potential as a biomarker or therapeutic target, driving ongoing interest in refining antibody reliability for mechanistic and diagnostic studies.
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