| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GRB2-associated-binding protein 3, GRB2-associated binder 3, Growth factor receptor bound protein 2-associated protein 3, GAB3 |
| Entrez GeneID | 139716 |
| WB Predicted band size | 65.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GAB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human GAB3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GAB3 (N-term)抗体的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *GAB3 regulates macrophage differentiation and inflammatory response via SHP-2 interaction*
**作者**: Chen L, et al.
**摘要**: 本研究利用GAB3 (N-term)抗体通过Western blot和免疫共沉淀技术，揭示了GAB3在巨噬细胞分化中通过其N端结构域与SHP-2蛋白的相互作用，调控TLR4信号通路和炎症因子分泌的机制。
2. **文献名称**: *N-terminal phosphorylation of GAB3 promotes breast cancer metastasis through PI3K/AKT activation*
**作者**: Wang Y, et al.
**摘要**: 文章使用GAB3 (N-term)特异性抗体验证了GAB3在乳腺癌细胞中的表达定位，发现其N端磷酸化通过激活PI3K/AKT信号轴增强肿瘤细胞的侵袭转移能力，为靶向治疗提供新思路。
3. **文献名称**: *Development and validation of a GAB3-specific monoclonal antibody for neurological disorder studies*
**作者**: Smith J, et al.
**摘要**: 本研究报道了一种针对GAB3 N端的单克隆抗体的开发与验证，证明其在小鼠脑组织切片中的高特异性染色能力，并初步探索了GAB3在阿尔茨海默病模型中的表达变化。
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**备注**：以上文献为示例性内容，实际引用时请通过 **PubMed** 或 **Google Scholar** 检索关键词：
`"GAB3 antibody N-terminal"`、`"GAB3 signaling"`、`"GRB2-associated binding protein 3"`，并筛选近年研究。
The GAB3 (N-term) antibody is designed to target the N-terminal region of the GRB2-associated binding protein 3 (GAB3), a scaffolding protein involved in intracellular signaling pathways. GAB3 belongs to the GAB family (GAB1. GAB2. GAB3), which interacts with adaptor proteins like GRB2 to mediate signals from receptor tyrosine kinases (RTKs) and cytokine receptors. The N-terminal region of GAB3 contains critical binding motifs, including a pleckstrin homology (PH) domain that facilitates membrane localization by binding phosphoinositides, and proline-rich sequences that recruit SH3 domain-containing partners. This antibody is commonly used to detect full-length GAB3 in applications such as Western blotting, immunohistochemistry, or immunofluorescence, helping researchers study its expression and localization in cells or tissues.
GAB3 is implicated in immune cell signaling, particularly in macrophages and neutrophils, and has been linked to pathologies like cancer and inflammatory diseases. Its role in activating PI3K/AKT and MAPK pathways underscores its importance in cell proliferation, survival, and differentiation. The N-term-specific antibody aids in distinguishing GAB3 from homologous family members (e.g., GAB1/GAB2) due to sequence divergence in the N-terminal region. Studies using this antibody have explored GAB3's involvement in oncogenic signaling, tumor microenvironment interactions, and potential therapeutic targeting. Validation via knockout controls or siRNA-mediated depletion ensures specificity in experimental models.
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