| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Death-associated protein kinase 1, DAP kinase 1, Dapk1 |
| Entrez GeneID | 69635 |
| WB Predicted band size | 161.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Dapk1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-412 amino acids from the N-terminal region of mouse Dapk1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于小鼠DAPK1 (N-term)抗体的示例参考文献(注：部分文献为虚构示例，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*DAPK1-mediated autophagy in neuronal cell death*
**作者**：Smith A, et al.
**摘要**：本研究利用N端特异性小鼠DAPK1抗体，通过Western blot和免疫荧光技术，证实DAPK1在神经元缺氧模型中激活自噬通路，并促进 caspase-3依赖性凋亡。
2. **文献名称**：*Role of DAPK1 in ischemic brain injury: Insights from a murine model*
**作者**：Chen L, et al.
**摘要**：通过N-terminal DAPK1抗体检测小鼠脑缺血后皮层区DAPK1蛋白表达上调，并发现其与ERK信号通路相互作用，提示其在神经退行性病变中的潜在治疗靶点。
3. **文献名称**：*DAPK1 interacts with microtubule-associated proteins via its N-terminal domain*
**作者**：Gomez-Ramos P, et al.
**摘要**：该研究采用N端特异性抗体进行免疫共沉淀(Co-IP)，发现DAPK1与微管结合蛋白Tau直接互作，为阿尔茨海默病中异常磷酸化Tau的积累机制提供了新证据。
4. **文献名称**：*Epigenetic silencing of DAPK1 in colorectal cancer*
**作者**：Wang Y, et al.
**摘要**：利用小鼠DAPK1 N端抗体分析结直肠癌组织样本，发现DAPK1启动子甲基化导致蛋白表达缺失，且与患者预后不良显著相关，支持其作为肿瘤抑制因子的作用。
(注：以上文献为示例性质，实际引用需以真实发表文献为准。)
The Mouse Dapk1 (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the N-terminal region of death-associated protein kinase 1 (DAPK1) in mouse samples. DAPK1 is a calcium/calmodulin-regulated serine/threonine kinase involved in diverse cellular processes, including apoptosis, autophagy, inflammation, and membrane blebbing. It plays critical roles in tumor suppression, neurodegeneration, and immune regulation by interacting with signaling pathways such as p53. NF-κB, and cytoskeletal components. The N-terminal region of DAPK1 contains the kinase domain and regulatory motifs essential for its catalytic activity and interactions with binding partners.
This antibody is widely used in research applications like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous DAPK1 expression levels or localization in mouse tissues or cell lines. Its specificity for the N-terminus ensures recognition of full-length DAPK1. distinguishing it from splice variants or truncated forms. Studies utilizing this antibody have contributed to understanding DAPK1's role in diseases such as cancer, Alzheimer's, and stroke, where its dysregulation is implicated. Validation often includes knockout controls or peptide blocking to confirm target specificity. Researchers rely on this tool to explore DAPK1's molecular mechanisms and therapeutic potential.
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