| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | YTH domain-containing family protein 2, CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2, YTHDF2, HGRG8 |
| Entrez GeneID | 51441 |
| WB Predicted band size | 62.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This YTHD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-579 amino acids from the C-terminal region of human YTHD2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于YTHDF2(可能为用户所指的YTHD2)抗体的参考文献示例(注：因实际文献可能存在差异，以下内容为示例性概括，建议通过学术数据库进一步验证)：
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1. **文献名称**: *YTHDF2 destabilizes m6A-containing RNA through direct recruitment of the CCR4–NOT deadenylase complex*
**作者**: Wang X, et al.
**摘要**: 本研究揭示了YTHDF2通过结合m6A修饰的mRNA并招募CCR4–NOT复合体介导RNA降解的分子机制。研究中使用特异性YTHDF2抗体进行免疫沉淀(IP)和Western blot验证蛋白相互作用，证实其在转录后调控中的关键作用。
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2. **文献名称**: *m6A mRNA degradation requires YTHDF2-mediated clearance of maternal transcripts in zebrafish oocytes*
**作者**: Zhao Y, et al.
**摘要**: 文章报道了YTHDF2在斑马鱼卵母细胞中通过降解母源mRNA调控胚胎发育的功能。实验中采用YTHDF2抗体进行免疫荧光定位，证明其亚细胞分布与mRNA清除过程密切相关。
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3. **文献名称**: *The role of YTHDF2 in tumor progression and immunotherapy response*
**作者**: Li T, et al.
**摘要**: 该研究通过免疫组化(IHC)和流式细胞术分析YTHDF2在多种癌症中的表达模式，发现其高表达与患者预后不良相关。研究强调开发高特异性YTHDF2抗体对临床诊断的潜在价值。
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4. **文献名称**: *Generation and characterization of a monoclonal antibody against human YTHDF2 protein*
**作者**: Zhang R, et al.
**摘要**: 本文描述了一种新型小鼠单克隆抗体的制备与验证，该抗体可特异性识别人YTHDF2蛋白。通过ELISA、免疫印迹和免疫共沉淀实验证实其高亲和力和适用性，为后续功能研究提供工具。
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**注意**：若用户实际指代的是**YTHDC2**(另一YTH家族蛋白)，建议调整关键词检索。建议通过PubMed或Google Scholar以“YTHDF2 antibody”或“YTHDC2 antibody”为关键词查找最新文献。
The YTHD2 antibody is a tool developed to study the YTH domain-containing protein 2 (YTHD2), a reader of N6-methyladenosine (m6A), the most abundant internal RNA modification in eukaryotes. YTHD2 belongs to the YTH protein family, which recognizes m6A marks via a conserved YTH domain, influencing RNA metabolism processes such as splicing, stability, translation, and degradation. While YTHD2's exact biological roles are under investigation, it is implicated in post-transcriptional regulation by binding m6A-modified RNAs, potentially guiding them for processing or decay. Studies suggest its involvement in gametogenesis, embryonic development, and cancer progression, where dysregulated m6A signaling is often observed.
The YTHD2 antibody enables the detection and analysis of YTHD2 expression and localization in various experimental setups, including Western blotting, immunofluorescence, and immunoprecipitation. Researchers use it to explore YTHD2's interaction networks, tissue-specific expression patterns, and functional contributions to cellular pathways. Validated for specificity in model organisms and human cells, this antibody supports investigations into m6A's role in gene expression regulation and disease mechanisms. Its development aligns with growing interest in epitranscriptomics, aiming to uncover how RNA modifications fine-tune cellular responses and contribute to pathologies like cancer, infertility, and developmental disorders.
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