| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EphB1 |
| Uniprot No | P54762-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-540aa |
| 氨基酸序列 | MALDYLLLLL LASAVAAMEE TLMDTRTATA ELGWTANPAS GWEEVSGYDE NLNTIRTYQV CNVFEPNQNN WLLTTFINRR GAHRIYTEMR FTVRDCSSLP NVPGSCKETF NLYYYETDSV IATKKSAFWS EAPYLKVDTI AADESFSQVD FGGRLMKVNT EVRSFGPLTR NGFYLAFQDY GACMSLLSVR VFFKKCPSIV QNFAVFPETM TGAESTSLVI ARGTCIPNAE EVDVPIKLYC NGDGEWMVPI GRCTCKPGYE PENSVACKAC PAGTFKASQE AEGCSHCPSN SRSPAEASPI CTCRTGYYRA DFDPPEVACT SVPSGPRNVI SIVNETSIIL EWHPPRETGG RDDVTYNIIC KKCRADRRSC SRCDDNVEFV PRQLGLTECR VSISSLWAHT PYTFDIQAIN GVSSKSPFPP QHVSVNITTN QAAPSTVPIM HQVSATMRSI TLSWPQPEQP NGIILDYEIR YYEKEHNEFN SSMARSQTNT ARIDGLRPGM VYVVQVRART VAGYGKFSGK MCFQTLTDDD YKSELREQLP |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于EphB1重组蛋白的研究文献概览(虚构示例，仅用于演示格式)：
1. **文献名称**：*"Recombinant EphB1-Fc fusion protein inhibits cell migration by binding Ephrin-B ligands"*
**作者**：Smith J, et al.
**摘要**：研究利用重组EphB1胞外段与Fc融合蛋白(EphB1-Fc)，证明其可通过竞争性结合Ephrin-B配体，抑制肿瘤细胞的迁移能力，并揭示了其在调控细胞粘附信号通路中的作用。
2. **文献名称**：*"Structural analysis of EphB1 kinase domain using recombinant protein expression in HEK293 cells"*
**作者**：Li X, et al.
**摘要**：通过HEK293细胞系统表达并纯化EphB1激酶结构域重组蛋白，结合X射线晶体学解析其三维结构，阐明了其自磷酸化机制及与ATP类似物的结合模式。
3. **文献名称**：*"EphB1 recombinant protein suppresses colorectal cancer metastasis via reverse signaling activation"*
**作者**：Wang Y, et al.
**摘要**：体外实验显示，可溶性EphB1重组蛋白通过激活Ephrin-B2的反向信号传导，抑制结直肠癌细胞侵袭性，并降低小鼠模型中肝转移的发生率。
4. **文献名称**：*"Purification and functional characterization of EphB1 ectodomain from insect cell expression system"*
**作者**：Garcia R, et al.
**摘要**：采用杆状病毒-昆虫细胞系统高效表达EphB1胞外域重组蛋白，优化纯化流程后验证其与Ephrin-B1的高亲和力结合，为后续受体配体互作研究提供工具。
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**注**：以上文献信息为模拟生成，实际文献需通过PubMed或Google Scholar检索关键词如“EphB1 recombinant protein”“EphB1 expression”获取。
**Background of EphB1 Recombinant Protein**
EphB1. a member of the Eph receptor tyrosine kinase family, plays critical roles in cell-cell communication, tissue patterning, and developmental processes. These receptors interact with membrane-bound ephrin ligands, triggering bidirectional signaling pathways that regulate cell adhesion, migration, and spatial organization. EphB1 is composed of an extracellular domain for ephrin-B ligand binding, a transmembrane region, and an intracellular tyrosine kinase domain for downstream signal transduction.
Recombinant EphB1 protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the receptor or its domains (e.g., extracellular regions for ligand-binding studies). This protein serves as a vital tool for investigating EphB1-ephrin interactions, signaling mechanisms, and its roles in physiological and pathological contexts.
Research highlights EphB1's involvement in neural development, synaptic plasticity, and angiogenesis. Dysregulation of EphB1 is linked to cancer progression, neurodegenerative diseases, and vascular disorders. For example, EphB1 suppresses tumor invasiveness in colorectal cancer but may promote metastasis in other cancers, underscoring its context-dependent functions.
The recombinant protein enables structure-function studies, drug screening for EphB1-targeted therapies, and mechanistic exploration of its cross-talk with pathways like Wnt or integrin signaling. Its applications extend to developing diagnostic tools or biologics aimed at modulating EphB1 activity in disease. Overall, EphB1 recombinant protein is indispensable for unraveling the receptor's multifaceted roles and therapeutic potential.
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