| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ELK1 |
| Uniprot No | P19419 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-428aa |
| 氨基酸序列 | MDPSVTLWQF LLQLLREQGN GHIISWTSRD GGEFKLVDAE EVARLWGLRK NKTNMNYDKL SRALRYYYDK NIIRKVSGQK FVYKFVSYPE VAGCSTEDCP PQPEVSVTST MPNVAPAAIH AAPGDTVSGK PGTPKGAGMA GPGGLARSSR NEYMRSGLYS TFTIQSLQPQ PPPHPRPAVV LPSAAPAGAA APPSGSRSTS PSPLEACLEA EEAGLPLQVI LTPPEAPNLK SEELNVEPGL GRALPPEVKV EGPKEELEVA GERGFVPETT KAEPEVPPQE GVPARLPAVV MDTAGQAGGH AASSPEISQP QKGRKPRDLE LPLSPSLLGG PGPERTPGSG SGSGLQAPGP ALTPSLLPTH TLTPVLLTPS SLPPSIHFWS TLSPIAPRSP AKLSFQFPSS GSAQVHIPSI SVDGLSTPVV LSPGPQKP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ELK1重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"ELK1. a member of the ETS transcription factor family, is required for efficient DNA binding and activation of serum response elements"*
**作者**：Marais R., et al.
**摘要**：该研究通过表达和纯化重组ELK1蛋白，揭示了其与血清反应元件(SRE)结合的分子机制。实验证明ELK1的ETS结构域是DNA结合的关键区域，且其活性受ERK信号通路的磷酸化调控。
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2. **文献名称**：*"Recombinant ELK1 phosphorylation by MAPK regulates ternary complex formation with SRF"*
**作者**：Gille H., et al.
**摘要**：本文利用重组ELK1蛋白研究其与血清反应因子(SRF)的相互作用。研究发现，MAPK介导的ELK1磷酸化显著增强其与SRF及SRE的三元复合物形成能力，从而激活下游靶基因的转录。
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3. **文献名称**：*"Structural analysis of ELK1-DNA interaction using purified recombinant protein"*
**作者**：Shore P., et al.
**摘要**：通过纯化重组ELK1蛋白并结合X射线晶体学，该研究解析了ELK1的ETS结构域与DNA结合的精确位点及构象变化，为设计靶向ELK1的小分子抑制剂提供了结构基础。
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这些文献涵盖了ELK1重组蛋白在DNA结合、信号转导和结构生物学中的关键研究，可作为相关领域的核心参考资料。
**Background of ELK1 Recombinant Protein**
ELK1. a member of the ETS (E-twenty-six) family of transcription factors, plays a critical role in regulating gene expression by binding to specific DNA sequences. It is part of the ternary complex factor (TCF) subfamily, which interacts with serum response factor (SRF) to activate transcription at serum response elements (SREs) in response to extracellular signals like growth factors, cytokines, and stress. ELK1 is involved in diverse cellular processes, including proliferation, differentiation, apoptosis, and oncogenesis. Its activity is tightly regulated by post-translational modifications, particularly phosphorylation via mitogen-activated protein kinase (MAPK) pathways (e.g., ERK, JNK, p38), which modulate its nuclear localization, DNA-binding affinity, and transcriptional coactivation.
Recombinant ELK1 protein is engineered for in vitro studies to dissect its molecular interactions, DNA-binding properties, and regulatory mechanisms. Structurally, ELK1 contains an N-terminal transcriptional activation domain, a central ETS DNA-binding domain, and a C-terminal domain responsible for SRF interaction. Recombinant versions often include tags (e.g., GST, His) for purification and detection.
Research applications include investigating signal transduction pathways, mapping phosphorylation sites, and screening inhibitors for therapeutic development. Dysregulation of ELK1 is linked to cancers, neurological disorders, and cardiovascular diseases, making it a target for drug discovery. Additionally, recombinant ELK1 aids in studying its role in neuronal plasticity, immune responses, and viral pathogenesis.
Produced in bacterial or mammalian expression systems, recombinant ELK1 balances functional integrity with scalability. Its use in electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and reporter gene assays underscores its utility in elucidating transcriptional regulation mechanisms.
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