| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPHA3 |
| Uniprot No | P29320 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-541aa |
| 氨基酸序列 | ELIPQPSNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQVCNVMDHSQNNWLRTNWVPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHGVKFREHQFTKIDTIAADESFTQMDLGDRILKLNTEIREVGPVNKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCSCNAGYEERGFMCQACRPGFYKALDGNMKCAKCPPHSSTQEDGSMNCRCENNYFRADKDPPSMACTRPPSSPRNVISNINETSVILDWSWPLDTGGRKDVTFNIICKKCGWNIKQCEPCSPNVRFLPRQFGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSSPPRQFAAVSITTNQAAPSPVLTIKKDRTSRNSISLSWQEPEHPNGIILDYEVKYYEKQEQETSYTILRARGTNVTISSLKPDTIYVFQIRARTAAGYGTNSRKFEFETSPDSFSISGESSQ |
| 预测分子量 | 61.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EPHA3重组蛋白的3篇参考文献示例(文献信息为虚构模拟，仅供格式参考)：
---
1. **文献名称**：*Structural Insights into EPHA3 Receptor Activation by Recombinant Ectodomain*
**作者**：Smith J, et al.
**摘要**：本研究通过昆虫细胞表达系统纯化EPHA3重组胞外域蛋白，结合X射线晶体学解析其与配体ephrin-A5结合的构象变化，揭示了EPHA3激活的关键结构域和分子机制。
---
2. **文献名称**：*EPHA3 Recombinant Protein Suppresses Lung Cancer Invasion via Downregulating EMT Pathways*
**作者**：Chen L, et al.
**摘要**：利用哺乳动物HEK293细胞表达的EPHA3-Fc重组蛋白处理肺癌细胞系，发现其通过抑制上皮-间质转化(EMT)相关信号(如Slug和β-catenin)，显著降低肿瘤细胞的迁移和侵袭能力。
---
3. **文献名称**：*Development of a High-Yield Escherichia coli Expression System for Soluble EPHA3 Kinase Domain*
**作者**：Kumar R, et al.
**摘要**：优化大肠杆菌表达条件(如低温诱导、融合标签筛选)，成功获得高纯度可溶性EPHA3激酶结构域重组蛋白，并验证其体外激酶活性，为药物筛选提供可靠工具。
---
4. **文献名称**：*EPHA3 Recombinant Protein Modulates Angiogenesis in Zebrafish Models*
**作者**：Garcia M, et al.
**摘要**：通过斑马鱼胚胎实验证明，外源性添加EPHA3重组蛋白可干扰血管内皮细胞导向，抑制VEGF介导的血管新生，提示其在抗血管生成治疗中的潜在应用。
---
(注：以上文献及摘要均为模拟生成，实际研究中请通过PubMed/SCI数据库检索真实文献。)
Eph receptor A3 (EPHA3) is a member of the Eph family of receptor tyrosine kinases, which play critical roles in cell-cell communication, tissue patterning, and developmental processes. Structurally, EPHA3 consists of an extracellular region with a ligand-binding domain, a cysteine-rich motif, and two fibronectin type III repeats, followed by a transmembrane helix and a cytoplasmic tyrosine kinase domain. It primarily interacts with membrane-bound ephrin-A ligands, initiating bidirectional signaling pathways that regulate cell adhesion, migration, and spatial organization. Dysregulation of EPHA3 has been implicated in various cancers, neurodegenerative diseases, and cardiovascular disorders, making it a potential therapeutic target.
Recombinant EPHA3 proteins are engineered in vitro to study its biological functions and interactions. These proteins are typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as glycosylation. The recombinant form often includes the extracellular domain fused to tags like Fc or His for purification and detection. Researchers utilize EPHA3 recombinant proteins to investigate ligand-receptor binding kinetics, signal transduction mechanisms, and downstream effects in cellular assays. They also serve as critical tools for screening small-molecule inhibitors or antibodies aimed at modulating EPHA3 activity in disease contexts.
Quality control of recombinant EPHA3 involves verifying purity (>95% by SDS-PAGE), endotoxin levels, and functional validation through ligand-binding assays (e.g., ELISA or surface plasmon resonance). Its bioactivity is confirmed via phosphorylation assays or cell-based migration/invasion models. By enabling precise mechanistic studies, recombinant EPHA3 advances our understanding of its role in health and disease, supporting drug discovery and biomarker development efforts.
×
