| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42 |
| Entrez GeneID | 9525 |
| WB Predicted band size | 49.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This VPS4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-332 amino acids from the C-terminal region of human VPS4B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于VPS4B抗体的3篇参考文献示例(文献信息为模拟，实际需根据真实文献调整)：
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1. **文献名称**: "VPS4B dysfunction impairs lysosomal degradation and promotes exosome secretion in colorectal cancer"
**作者**: Zhang Y, et al.
**摘要**: 研究使用VPS4B特异性抗体进行Western blot和免疫荧光，发现VPS4B缺失导致溶酶体降解通路异常，促进结直肠癌细胞外泌体分泌，加速肿瘤转移。
2. **文献名称**: "ESCRT-III protein VPS4B regulates cardiomyocyte cytokinesis via modulating endosomal trafficking"
**作者**: Li H, et al.
**摘要**: 通过VPS4B抗体阻断实验，揭示其在心肌细胞胞质分裂中的作用，证明VPS4B通过调控内吞体运输影响心脏发育缺陷。
3. **文献名称**: "VPS4B mutations disrupt extracellular vesicle biogenesis and enhance HIV-1 budding efficiency"
**作者**: Smith J, et al.
**摘要**: 利用VPS4B抗体进行免疫沉淀和电镜分析，发现VPS4B基因突变会增强HIV-1病毒出芽能力，为抗病毒治疗提供新靶点。
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**注**：以上文献为示例，实际引用时请以真实文献为准，建议通过PubMed或Google Scholar以关键词“VPS4B antibody”、“VPS4B function”检索近年研究。
The VPS4B (Vacuolar Protein Sorting 4 Homolog B) antibody is a tool used to study the VPS4B protein, a critical component of the Endosomal Sorting Complex Required for Transport (ESCRT) pathway. VPS4B belongs to the AAA+ ATPase family and plays a key role in membrane remodeling processes, such as cytokinesis, multivesicular body (MVB) formation, viral budding, and autophagy. It hydrolyzes ATP to disassemble and recycle ESCRT-III polymers, enabling membrane scission or cargo sorting. Dysregulation of VPS4B is linked to cancer, neurodegenerative disorders, and infections (e.g., HIV-1).
The VPS4B antibody is widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Both monoclonal and polyclonal variants exist, often validated for specificity using knockout controls. Studies employing this antibody have revealed insights into ESCRT-mediated membrane dynamics, cellular stress responses, and disease mechanisms. Its application helps elucidate how VPS4B dysfunction contributes to pathologies, offering potential therapeutic targets. Commercial antibodies typically cite reactivity across species (human, mouse, rat) and provide data on recommended dilutions and experimental conditions.
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