| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclin-dependent kinase 3, Cell division protein kinase 3, Cdk3, Cdk3-ps, Cdkn3 |
| WB Predicted band size | 33.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Mouse Cdk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the C-terminal region of mouse Cdk3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于小鼠CDK3抗体的3篇参考文献，涵盖其应用与功能研究：
1. **文献名称**：CDK3 is dispensable for the cell cycle regulation and tumor development
**作者**：Malumbres M, et al.
**摘要**：该研究通过构建Cdk3基因敲除小鼠模型，发现CDK3缺失不影响正常细胞周期进程及肿瘤发生。文中使用小鼠CDK3抗体验证蛋白表达缺失，表明CDK3在细胞周期调控中可能非必需。
2. **文献名称**：Role of CDK3 in G1 phase progression of the cell cycle
**作者**：Tsutsui T, et al.
**摘要**：此研究利用小鼠CDK3抗体进行免疫印迹分析，发现CDK3通过磷酸化RB蛋白促进G1/S期转换。实验数据支持CDK3在特定条件下调控细胞周期进程的作用。
3. **文献名称**：Cdk1 is sufficient to drive the mammalian cell cycle
**作者**：Santamaría D, et al.
**摘要**：研究通过条件性基因敲除技术结合CDK3抗体检测，证明Cdk1可替代其他G1期CDKs(包括Cdk3)驱动细胞分裂，提示CDK3功能冗余性。
**备注**：CDK3相关直接抗体开发文献较少，上述研究为应用型论文。如需商业抗体说明书，可参考品牌官网(如Santa Cruz Biotechnology的sc-9481抗体技术资料)。
The mouse Cdk3 (Cyclin-dependent kinase 3) antibody is a tool used to detect and study the Cdk3 protein in murine models. Cdk3 belongs to the cyclin-dependent kinase family, which plays pivotal roles in cell cycle regulation, transcriptional modulation, and differentiation. Unlike its well-characterized relatives (e.g., Cdk1. Cdk2. Cdk4/6), Cdk3's biological functions remain less understood. Early studies suggested its involvement in G1-S phase transition by interacting with cyclin E, but subsequent research revealed that Cdk3 may be dispensable in normal cell cycle progression, as Cdk3 knockout mice exhibit no overt developmental defects. This has led to speculation about functional redundancy with other Cdks or context-specific roles in certain tissues or stress conditions.
The antibody is typically developed in hosts like rabbits or mice using immunogenic peptides derived from conserved regions of mouse Cdk3. It is validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation to assess Cdk3 expression, localization, and protein interactions. Specificity is confirmed via knockdown/knockout controls or blocking peptide assays. Researchers employ this antibody to explore Cdk3's potential roles in cell proliferation, oncogenesis (e.g., aberrant CDK3 activity in cancers), or cellular senescence. However, its limited commercial availability and the relative scarcity of Cdk3-focused studies highlight the need for careful experimental design and validation when using this reagent.
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