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| 纯度 | >97%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCL3L1 |
| Uniprot No | P16619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-93aa |
| 氨基酸序列 | APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA |
| 预测分子量 | 7.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCL3L1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*CCL3L1-CCR5 interaction modulates HIV-1 infection*
**作者**：Nibbs, R. et al.
**摘要**：该研究通过重组CCL3L1蛋白探究其与CCR5受体的结合特性，发现高浓度重组CCL3L1可竞争性抑制HIV-1病毒进入CD4+ T细胞，揭示了其作为潜在抗病毒治疗分子的机制。
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2. **文献名称**：*Structural and functional analysis of CCL3L1 polymorphisms in inflammatory disease*
**作者**：Smith, J.P. & Rostapshov, A.
**摘要**：利用重组CCL3L1蛋白进行结构建模和体外趋化实验，发现特定基因多态性影响蛋白与受体CCR1/CCR5的结合能力，解释了其在类风湿性关节炎等炎症疾病中的调控作用。
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3. **文献名称**：*CCL3L1 copy number variation alters monocyte migration in vitro*
**作者**：Townsend, M.J. et al.
**摘要**：通过表达纯化不同基因拷贝数的重组CCL3L1蛋白，证明其浓度梯度显著影响单核细胞的迁移效率，提示CCL3L1拷贝数差异可能导致个体间免疫应答的异质性。
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注：以上文献信息为示例性概括，实际引用需根据具体论文内容调整。建议通过PubMed或Web of Science以关键词“CCL3L1 recombinant protein”检索最新文献。
CCL3L1 is a variant of the C-C motif chemokine ligand 3 (CCL3), encoded by the *CCL3L1* gene located within a segmental duplication on chromosome 17q12. This protein belongs to the CC chemokine family, which plays critical roles in immune regulation by recruiting leukocytes to sites of inflammation or infection. CCL3L1 shares structural and functional similarities with CCL3. both binding to chemokine receptors CCR1. CCR3. and CCR5. However, CCL3L1 exhibits enhanced binding affinity to CCR5. a coreceptor for HIV-1 entry, making it a key focus in HIV pathogenesis research.
The *CCL3L1* gene is copy-number variable, with population-specific differences influencing disease susceptibility. Lower copy numbers have been associated with increased HIV-1 infection risk and accelerated disease progression, though findings remain debated due to population heterogeneity. Recombinant CCL3L1 is produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography (e.g., His-tag) and rigorous quality controls (SDS-PAGE, endotoxin testing). Its bioactivity is often validated through chemotaxis assays using CCR5-expressing cells.
In research, recombinant CCL3L1 serves as a tool to study CCR5-mediated signaling, immune cell migration, and viral entry mechanisms. It is also explored for therapeutic potential, including blocking HIV-1 infection by competitively inhibiting viral binding to CCR5. Additionally, its role in inflammatory diseases, such as rheumatoid arthritis and atherosclerosis, underscores its broader relevance in immunopathology. Despite functional overlaps with CCL3. CCL3L1's unique genetic variability and receptor interactions highlight its distinct biological and clinical significance.
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