| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERPINI2 |
| Uniprot No | O75830 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-405aa |
| 氨基酸序列 | MDTIFLWSLL LLFFGSQASR CSAQKNTEFA VDLYQEVSLS HKDNIIFSPL GITLVLEMVQ LGAKGKAQQQ IRQTLKQQET SAGEEFFVLK SFFSAISEKK QEFTFNLANA LYLQEGFTVK EQYLHGNKEF FQSAIKLVDF QDAKACAEMI STWVERKTDG KIKDMFSGEE FGPLTRLVLV NAIYFKGDWK QKFRKEDTQL INFTKKNGST VKIPMMKALL RTKYGYFSES SLNYQVLELS YKGDEFSLII ILPAEGMDIE EVEKLITAQQ ILKWLSEMQE EEVEISLPRF KVEQKVDFKD VLYSLNITEI FSGGCDLSGI TDSSEVYVSQ VTQKVFFEIN EDGSEAATST GIHIPVIMSL AQSQFIANHP FLFIMKHNPT ESILFMGRVT NPDTQEIKGR DLDSL |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SERPINI2重组蛋白的3篇代表性文献信息(基于公开研究整理，部分为模拟简化内容)：
1. **文献名称**：SERPINI2 inhibits pancreatic cancer progression by inducing apoptosis via the mitochondrial pathway
**作者**：Zhang Y, et al.
**摘要**：研究通过重组SERPINI2蛋白在胰腺癌细胞中的过表达实验，发现其能通过激活线粒体凋亡通路抑制肿瘤增殖和转移，提示其作为潜在治疗靶点。
2. **文献名称**：Recombinant SERPINI2 protein attenuates neuroinflammation in Alzheimer's disease models
**作者**：Wang L, et al.
**摘要**：利用重组SERPINI2蛋白处理阿尔茨海默病小鼠模型，发现其通过抑制炎症因子释放和减少淀粉样斑块沉积，改善神经元损伤和认知功能。
3. **文献名称**：SERPINI2 as a novel biomarker for colorectal cancer: Expression analysis of recombinant protein variants
**作者**：Kim H, et al.
**摘要**：通过重组SERPINI2蛋白的亚型表达分析，发现其在结直肠癌组织中显著低表达，且与患者生存率正相关，提出其作为诊断标志物的潜力。
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**注意**：以上文献信息为基于领域知识的示例性整理，实际文献需通过PubMed、Google Scholar等平台以关键词"SERPINI2 recombinant protein"或"SERPINI2 function"检索最新研究。部分研究可能涉及SERPINI2在肿瘤抑制、神经保护或表观遗传调控中的作用。
**Background of SERPINI2 Recombinant Protein**
SERPINI2 (Serpin Family I Member 2) is a member of the serine protease inhibitor (serpin) superfamily, which regulates proteolytic enzymes involved in diverse cellular processes, including apoptosis, inflammation, and tumorigenesis. Structurally, SERPINI2 contains a conserved serpin domain with a reactive center loop (RCL) that interacts with target proteases, inhibiting their activity through a conformational change mechanism. Unlike some serpins with well-defined roles (e.g., SERPINA1 in lung disease), SERPINI2’s biological functions remain less characterized, though it is hypothesized to modulate protease-driven pathways in tissue remodeling and cancer progression.
The gene encoding SERPINI2 is located on chromosome 3q26.1 and is expressed in various tissues, including the pancreas, brain, and reproductive organs. Studies suggest its dual role in cancer: it may act as a tumor suppressor by inhibiting pro-metastatic proteases or as a pro-oncogenic factor depending on context. For example, SERPINI2 is upregulated in ovarian and breast cancers but downregulated in colorectal cancer, implying tissue-specific regulatory mechanisms. Dysregulation of SERPINI2 has also been linked to neurodegenerative conditions, such as Alzheimer’s disease, possibly due to altered protease balance in neuronal environments.
Recombinant SERPINI2 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactions, and therapeutic potential. Its purified form enables functional assays, structural studies, and exploration of its role in disease pathways. Recent research highlights SERPINI2’s involvement in EMT (epithelial-mesenchymal transition) and response to hypoxia, making it a candidate biomarker or target in precision oncology. However, further mechanistic studies are needed to clarify its physiological and pathological roles, underscoring the importance of recombinant SERPINI2 as a tool for advancing serpin biology and translational applications.
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