| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADAMDEC1 |
| Uniprot No | O15204 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 206-470aa |
| 氨基酸序列 | KSPEKEDFLRAQKYIDLYLVLDNAFYKNYNENLTLIRSFVFDVMNLLNVI YNTIDVQVALVGMEIWSDGDKIKVVPSASTTFDNFLRWHSSNLGKKIHDH AQLLSGISFNNRRVGLAASNSLCSPSSVAVIEAKKKNNVALVGVMSHELG HVLGMPDVPFNTKCPSGSCVMNQYLSSKFPKDFSTSCRAHFERYLLSQKP KCLLQAPIPTNIMTTPVCGNHLLEVGEDCDCGSPKECTNLCCEALTCKLK PGTDCGGDAPNHTTEVDHHHHHH |
| 预测分子量 | 51 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADAMDEC1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"ADAMDEC1 is a novel metalloprotease with a functional role in colorectal cancer"*
**作者**: Smith A, et al.
**摘要**: 该研究成功在大肠杆菌中表达了重组ADAMDEC1蛋白，并验证其金属蛋白酶活性。实验发现，ADAMDEC1在结肠癌组织中高表达，可能通过调节细胞外基质降解促进肿瘤侵袭。
2. **文献名称**: *"Recombinant ADAMDEC1 suppresses dendritic cell-mediated T cell activation via cleavage of CD86"*
**作者**: Li Y, et al.
**摘要**: 研究利用哺乳动物细胞系统表达并纯化ADAMDEC1重组蛋白，发现其能够切割树突状细胞表面的CD86分子，抑制T细胞活化，提示其在免疫调节中的潜在作用。
3. **文献名称**: *"Structural and functional characterization of the catalytic domain of ADAMDEC1"*
**作者**: Wang X, et al.
**摘要**: 通过昆虫表达系统获得ADAMDEC1催化结构域重组蛋白，解析其晶体结构，并揭示其底物特异性。研究提出ADAMDEC1可能通过独特的酶切机制参与炎症性疾病进程。
*注：以上文献信息为模拟示例，实际引用需以真实论文数据为准。建议通过PubMed或Web of Science检索最新研究。*
**Background of ADAMDEC1 Recombinant Protein**
ADAMDEC1 (A Disintegrin And Metalloproteinase Domain-Like Protein Decysin 1) is a member of the ADAM family of proteases, which are characterized by their multidomain structure and involvement in proteolytic processing, cell adhesion, and signaling. Unlike most ADAM proteins, ADAMDEC1 lacks transmembrane and cytoplasmic domains, suggesting it is a secreted enzyme. It is encoded by the *ADAMDEC1* gene, located on chromosome 8 in humans, and is primarily expressed in immune cells, particularly dendritic cells and macrophages, as well as in certain epithelial tissues.
Functionally, ADAMDEC1 exhibits metalloproteinase activity but with a distinct substrate specificity compared to other ADAMs. It has been implicated in immune regulation, inflammation, and tissue remodeling. Studies suggest it may cleave extracellular matrix components, chemokines, or cytokines, potentially modulating immune responses. For example, it may process pro-forms of cytokines like IL-1β or degrade chemokines to regulate leukocyte recruitment. Its role in diseases such as cancer, rheumatoid arthritis, and inflammatory bowel disease is under investigation, with some evidence linking its expression to tumor progression or anti-inflammatory effects.
Recombinant ADAMDEC1 protein is produced in vitro (e.g., in mammalian or insect cell systems) to study its biochemical properties, structure, and interactions. This tool enables research into its enzymatic mechanisms, substrate identification, and therapeutic potential. Due to its restricted expression pattern and unique enzymatic activity, ADAMDEC1 is considered a potential biomarker or drug target for immune-related disorders. However, its precise biological roles and regulatory pathways remain incompletely understood, necessitating further exploration.
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