| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PAOX; PAO; Peroxisomal N(1)-acetyl-spermine/spermidine oxidase; Polyamine oxidase |
| Entrez GeneID | 196743 |
| WB Predicted band size | Calculated MW: 56 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from PAOX . at AA range: 260-340 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于PAOX(多胺氧化酶)抗体的3篇参考文献概览，基于领域内典型研究方向的假设性整理：
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1. **文献名称**：*Development of a Monoclonal Antibody Against Human Polyamine Oxidase for Cancer Biomarker Studies*
**作者**：Smith A, et al.
**摘要**：本研究报道了一种针对人源PAOX蛋白的单克隆抗体的开发与验证。该抗体通过免疫印迹和免疫组化验证，证实可特异性识别多种癌细胞系中PAOX的表达水平，为癌症相关多胺代谢异常提供了检测工具。
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2. **文献名称**：*PAOX-Specific Antibody Reveals Its Role in Neuronal Redox Homeostasis*
**作者**：Zhang L, et al.
**摘要**：通过构建PAOX基因敲除小鼠模型，结合定制多克隆抗体的应用，揭示了PAOX在神经元氧化应激调控中的关键作用。抗体经ELISA和免疫荧光验证，支持其在脑组织中的特异性定位分析。
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3. **文献名称**：*Plant PAOX Antibody Applications in Abiotic Stress Response Analysis*
**作者**：Tanaka K, et al.
**摘要**：开发了一种植物来源PAOX的多克隆抗体，用于研究盐胁迫下水稻中多胺代谢通路的动态变化。抗体在植物组织提取物中表现出高亲和力，助力于蛋白表达定量及亚细胞定位研究。
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**备注**：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science以“PAOX antibody”、“polyamine oxidase immunoassay”等关键词检索最新文献，并关注抗体开发、验证或特定应用场景的研究。
**Background of PAOX Antibody**
The PAOX (Peroxisomal Acyl-CoA Oxidase 1) antibody is a tool used to detect and study the enzyme ACOX1. a key player in peroxisomal fatty acid β-oxidation. ACOX1 catalyzes the first rate-limiting step in breaking down very-long-chain fatty acids (VLCFAs) and branched-chain fatty acids, generating hydrogen peroxide as a byproduct. This enzyme is critical for lipid metabolism and energy homeostasis, particularly in the liver, kidneys, and brain.
Structurally, ACOX1 is a 70-75 kDa homodimeric protein encoded by the *ACOX1* gene. Dysregulation of ACOX1 is linked to peroxisomal disorders, such as adrenoleukodystrophy (ALD), and metabolic syndromes. Elevated ACOX1 levels have also been associated with oxidative stress, inflammation, and certain cancers, highlighting its dual role in health and disease.
PAOX antibodies are widely used in research to investigate peroxisomal function, lipid metabolism abnormalities, and related pathologies. They enable techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to visualize ACOX1 expression and localization in tissues or cell lines. These antibodies are vital for studying metabolic diseases, neurodegenerative conditions, and cancer mechanisms, offering insights into potential therapeutic targets. Commercial PAOX antibodies are typically validated for specificity and sensitivity, ensuring reliability in experimental and diagnostic applications.
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