| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C |
| Entrez GeneID | 5515 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PP2A-alpha. AA range:260-309 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于PP2A α抗体的3篇参考文献的简要信息(文献及摘要内容为示例，仅供参考)：
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1. **文献名称**：*Structural and functional analysis of PP2A α subunit phosphorylation*
**作者**：Chen et al.
**摘要**：该研究利用特异性PP2A α抗体，通过免疫印迹和质谱分析，揭示了PP2A α亚基在酪氨酸磷酸化修饰中的关键位点及其对酶活性的调控机制，为靶向PP2A的疾病治疗提供理论依据。
2. **文献名称**：*PP2A α expression correlates with poor prognosis in colorectal cancer*
**作者**：Wang et al.
**摘要**：通过免疫组化技术结合PP2A α抗体，研究发现结直肠癌组织中PP2A α蛋白表达水平显著下调，且与患者生存期缩短相关，提示其可能作为潜在预后标志物。
3. **文献名称**：*PP2A α regulates autophagy via mTOR signaling pathway*
**作者**：Li & Zhang
**摘要**：该研究使用PP2A α抗体进行免疫共沉淀实验，证实PP2A α通过与mTOR复合物相互作用调控自噬过程，为神经退行性疾病中的自噬异常机制提供新见解。
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**注意**：以上文献信息为模拟示例，实际引用时需通过PubMed或Web of Science等平台核对真实文献。建议检索关键词“PP2A alpha antibody”、“PP2A catalytic subunit”或结合研究领域(如癌症、神经科学)筛选近期论文。
**Background of PP2A Alpha Antibody**
Protein Phosphatase 2A (PP2A) is a highly conserved serine/threonine phosphatase critical for regulating diverse cellular processes, including cell cycle progression, apoptosis, and signal transduction. The PP2A holoenzyme typically consists of a catalytic subunit (C), a structural subunit (A), and a variable regulatory subunit (B). The catalytic subunit exists in two isoforms, PP2A alpha (Cα) and PP2A beta (Cβ), encoded by distinct genes. PP2A alpha, the focus of PP2A alpha-specific antibodies, is ubiquitously expressed and plays essential roles in maintaining cellular homeostasis.
PP2A alpha antibodies are immunological tools designed to detect and quantify the expression, post-translational modifications (e.g., phosphorylation), or interactions of the PP2A Cα subunit. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study PP2A alpha's involvement in diseases such as cancer, Alzheimer’s disease, and cardiac pathologies, where its dysregulation is often observed. For example, reduced PP2A alpha activity is linked to hyperphosphorylation of tau in neurodegenerative disorders.
Monoclonal or polyclonal PP2A alpha antibodies are validated for specificity to avoid cross-reactivity with the beta isoform. Their applications extend to exploring PP2A’s role as a tumor suppressor or therapeutic target, given its interactions with oncogenic signaling pathways. Researchers rely on these antibodies to dissect PP2A’s complex regulatory mechanisms and its potential in drug development.
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