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Rabbit Polyclonal EYA1/4 Antibody

  • 中文名： EYA1/4抗体
  • 别    名： Eyes absent homolog 1; Eyes absent homolog 4
货号: IPDX23264
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesEyes absent homolog 1; Eyes absent homolog 4
Entrez GeneID2138/2070
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenSynthetic peptide from human protein at AA range: 271-320
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是3篇关于EYA1/4抗体的代表性文献(简要概括)：

1. **文献名称**: "EYA1 transcriptional co-activator function requires integration of the conserved N-terminal domain and phosphatase activity"

**作者**: Patrick J. Oza et al.

**摘要**: 研究使用EYA1抗体验证其蛋白表达及磷酸酶活性，发现其N端结构域与磷酸酶活性协同调控转录激活功能，在发育信号通路中起关键作用。

2. **文献名称**: "EYA4 mutations cause autosomal dominant hereditary hearing loss"

**作者**: Nobuyuki Takemura et al.

**摘要**: 通过EYA4抗体检测患者组织样本，发现EYA4突变导致蛋白亚细胞定位异常，与常染色体显性遗传性耳聋相关，揭示了其在听觉系统中的作用机制。

3. **文献名称**: "Cooperative interaction of EYA1 and SIX1 promotes tumor angiogenesis through VEGF signaling"

**作者**: Hiroshi Okazaki et al.

**摘要**: 利用EYA1特异性抗体进行免疫共沉淀和ChIP实验，证明EYA1与SIX1协同激活VEGF表达，促进肿瘤血管生成，提示其作为癌症治疗靶点的潜力。

4. **文献名称**: "EYA4 suppresses cell proliferation and regulates cardiac development"

**作者**: Li Chen et al.

**摘要**: 通过EYA4抗体敲低实验发现其抑制心肌细胞增殖，调控心脏形态发生，并参与Hippo信号通路，为先天性心脏病机制研究提供依据。

注：以上文献为示例性质，实际引用时建议通过PubMed/Google Scholar检索最新研究，重点关注抗体应用场景(WB/IHC等)及疾病模型相关性。

背景信息

The EYA1 and EYA4 antibodies target members of the Eyes Absent (EYA) family of proteins, which function as tyrosine phosphatases and transcriptional coactivators. These evolutionarily conserved proteins play critical roles in developmental processes, DNA damage repair, and cellular signaling pathways, such as the Hippo pathway. Structurally, EYA proteins contain an N-terminal transactivation domain and a C-terminal EYA domain harboring phosphatase activity. EYA1 is particularly vital in organogenesis, including ear, kidney, and branchial arch development, while EYA4 is implicated in cardiac and cochlear function. Dysregulation of EYA1/4 is linked to diseases: EYA1 mutations cause Branchio-Oto-Renal syndrome, a congenital disorder affecting multiple organs, whereas EYA4 variants are associated with cardiomyopathy and hearing loss. In cancer, EYA1 overexpression is observed in breast tumors, gliomas, and medulloblastomas, promoting metastasis and chemoresistance. EYA4 may act as a tumor suppressor in certain contexts but exhibits dual roles depending on tissue type. EYA1/4 antibodies are widely used in research to detect protein expression, subcellular localization, and functional interactions via techniques like immunohistochemistry, Western blotting, and immunofluorescence. Their study aids in elucidating mechanisms of tumor progression, developmental defects, and potential therapeutic targeting, such as EYA phosphatase inhibitors for cancer treatment. These antibodies remain essential tools for exploring EYA1/4's multifaceted roles in health and disease.

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