| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Death domain containing protein p84N5; HPR1; hTREX84; Nuclear matrix protein p84; p84N5; THO complex 1; Tho1; Thoc1 |
| Entrez GeneID | 9984 |
| WB Predicted band size | Calculated MW: 76 kDa; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human Nuclear Matrix Protein p84 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于THO Complex Subunit 1(THOC1)抗体的参考文献示例，包括文献名称、作者及简化的摘要内容：
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1. **文献名称**：*THOC1 depletion induces apoptosis in hepatocellular carcinoma via R-loop accumulation and DNA damage*
**作者**：Li, X., Zhang, Y., & Chen, W.
**摘要**：本研究利用THOC1特异性抗体，通过Western blot和免疫组化技术，验证了THOC1在肝癌细胞中的高表达。敲低THOC1导致R-loop积累和DNA损伤，触发细胞凋亡，提示其作为肝癌治疗靶点的潜力。
2. **文献名称**：*The THO complex coordinates mRNA export with transcription and splicing*
**作者**：Reed, R., Strässer, K., & Hurt, E.
**摘要**：该文献通过THOC1抗体进行免疫沉淀和蛋白质组学分析，揭示了THO复合体在mRNA转录后输出中的核心作用。研究表明，THOC1与其他亚基协同调控mRNA的核质转运。
3. **文献名称**：*Developmental regulation of THOC1 in murine cortical neurons: Insights from immunofluorescence and RNA-seq*
**作者**：Smith, J., Lee, H., & Park, M.
**摘要**：利用THOC1抗体进行免疫荧光染色，发现其在神经元发育过程中动态表达，并参与突触形成。RNA-seq数据进一步支持THOC1与神经发育相关基因的剪接关联。
4. **文献名称**：*THOC1 interacts with viral RNA to facilitate influenza A replication*
**作者**：Wang, L., Zhou, Q., & Liu, S.
**摘要**：通过THOC1抗体进行Co-IP实验，证明其与流感病毒RNA结合，促进病毒复制。研究为宿主因子在病毒感染中的作用提供了新机制。
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注：上述文献为示例性内容，实际引用时需核实数据库(如PubMed、Google Scholar)中的真实文献并标注完整信息。
The THO complex subunit 1 (THOC1), also known as HPR1. is a critical component of the evolutionarily conserved THO complex, which plays a key role in coupling transcription with mRNA processing and nuclear export. THOC1 interacts with other THO subunits (e.g., THOC2. THOC5–7) and components of the mRNA export machinery to ensure efficient packaging of nascent transcripts into export-competent ribonucleoprotein particles (mRNPs). Dysregulation of THOC1 has been linked to transcriptional stress, defective mRNA export, and genomic instability.
Antibodies targeting THOC1 are widely used in research to study its expression, localization, and molecular interactions. These antibodies are typically validated for applications like Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) in models ranging from cultured cells to tissue samples. Many commercially available THOC1 antibodies are raised in rabbits or mice, with specificity confirmed via knockout validation or siRNA-mediated depletion.
Studies using THOC1 antibodies have shed light on its involvement in gene expression regulation, particularly in cancers and neurological disorders. For instance, reduced THOC1 levels correlate with poor prognosis in certain tumors, while its role in neuronal mRNA transport underscores its relevance in neurodegenerative diseases. Reliable THOC1 antibodies remain essential tools for dissecting the molecular mechanisms of mRNA metabolism and disease pathogenesis.
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