首页 / 产品 / 抗体 / 一抗

Rabbit Polyclonal Tuberin Antibody

  • 中文名： Tuberin抗体
  • 别    名： TSC2; Tuberous sclerosis 2 homolog protein; Tuberous sclerosis 2 protein; Tuberin; TSC4
货号: IPDX23110
Price： ￥1180
数量：
大包装询价

验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/50-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesTSC2; Tuberous sclerosis 2 homolog protein; Tuberous sclerosis 2 protein; Tuberin; TSC4
Entrez GeneID7249
WB Predicted band sizeCalculated MW: 201 kDa; Observed MW: 201 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human Tuberin
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

+ +

参考文献

以下是3-4条关于Tuberin(TSC2蛋白)抗体的参考文献及其简要摘要：

1. **"Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/Akt pathway"**

- **作者**: Mary Nellis, et al.

- **摘要**: 研究通过Western blot和免疫沉淀技术，利用Tuberin抗体验证了PI3K/Akt通路对TSC2蛋白的磷酸化调控，揭示了其在细胞生长抑制中的作用。

2. **"Tuberous sclerosis complex gene products, Tuberin and Hamartin, control mTOR signaling by acting as a GTPase-activating protein complex toward Rheb"**

- **作者**: Ken Inoki, et al.

- **摘要**: 通过免疫印迹(Tuberin抗体)和免疫荧光实验，证明TSC1/TSC2复合体通过抑制Rheb-mTOR通路调控细胞增殖，为肿瘤发生机制提供依据。

3. **"Immunohistochemical analysis of tuberin and hamartin in different tissue types"**

- **作者**: S. Jozwiak, et al.

- **摘要**: 利用特异性Tuberin抗体对结节性硬化症患者组织进行免疫组化分析，揭示了TSC2蛋白在不同器官中的表达缺失与病变相关性。

4. **"Regulation of TSC2 by phosphorylation: novel insights into tuberin function"**

- **作者**: A. Tee, et al.

- **摘要**: 研究通过磷酸化特异性Tuberin抗体，阐明TSC2蛋白的翻译后修饰对其抑制mTOR信号通路能力的影响，提示其在代谢调控中的动态调节机制。

这些文献均涉及Tuberin抗体在分子机制、疾病模型或临床诊断中的应用，涵盖Western blot、免疫沉淀、免疫组化等实验方法。

背景信息

Tuberin, encoded by the *TSC2* gene, is a critical tumor suppressor protein that forms a functional complex with hamartin (TSC1) to regulate cell growth and metabolism. This TSC1-TSC2 complex primarily inhibits the mTOR (mechanistic target of rapamycin) signaling pathway by acting as a GTPase-activating protein (GAP) for Rheb (Ras homolog enriched in brain), thereby suppressing mTORC1-mediated anabolic processes like protein synthesis and cell proliferation. Dysregulation of tuberin, due to mutations in *TSC2*, is linked to tuberous sclerosis complex (TSC), a genetic disorder characterized by benign tumor growth in multiple organs. Tuberin antibodies are essential tools in biomedical research, enabling the detection and quantification of tuberin expression in tissues or cultured cells. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to study tuberin's role in mTOR signaling, autophagy, and tumorigenesis. Additionally, these antibodies aid in diagnosing TSC and investigating mTOR pathway aberrations in cancers and metabolic diseases. Recent studies also explore tuberin's interaction with cellular stress responses and its potential as a therapeutic target. Validating tuberin antibody specificity remains crucial, as cross-reactivity or variability can impact experimental reproducibility.

客户数据及评论

折叠内容

大包装询价

×