| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAD; MADA |
| WB Predicted band size | 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human AMPD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于MBD3(Methyl-CpG Binding Domain Protein 3)抗体的模拟参考文献示例，供参考：
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1. **文献名称**：*MBD3 regulates pluripotency through nucleosome remodeling in embryonic stem cells*
**作者**：Kaji, K., et al.
**摘要**：本研究利用MBD3特异性抗体进行染色质免疫沉淀(ChIP)，揭示了MBD3作为NuRD复合体成员在小鼠胚胎干细胞多能性维持中的作用。发现MBD3通过调控核小体重塑影响Oct4和Nanog等关键基因的表达。
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2. **文献名称**：*Targeting MBD3 for epigenetic therapy in colorectal cancer*
**作者**：Zhang, L., et al.
**摘要**：通过Western blot和免疫组化分析，研究者发现结直肠癌组织中MBD3蛋白表达异常升高。使用MBD3抗体阻断其功能可抑制肿瘤细胞增殖，表明MBD3可能成为表观遗传治疗的潜在靶点。
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3. **文献名称**：*MBD3 cooperates with DNA methylation to maintain T cell identity*
**作者**：Burgold, T., et al.
**摘要**：利用MBD3敲除模型及特异性抗体，研究证明MBD3与DNA甲基化协同调控T细胞分化相关基因的沉默，维持免疫细胞功能稳定性。
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注：以上内容为模拟文献，实际引用时请以真实发表的论文为准。若需具体文献，建议在PubMed等数据库中检索关键词“MBD3 antibody”或联系相关领域研究者获取。
The MDB3 antibody is designed to target the Methyl-CpG-binding domain protein 3 (MBD3), a key component of the NuRD (Nucleosome Remodeling and Deacetylation) complex involved in epigenetic regulation. MBD3 binds to methylated DNA regions, facilitating chromatin remodeling and gene silencing. Unlike other MBD family members, MBD3 lacks strong methyl-CpG binding affinity in isolation but contributes to NuRD’s function in transcriptional repression, cell differentiation, and genome stability.
Discovered in the late 1990s, MBD3 is evolutionarily conserved and plays critical roles in embryonic development, stem cell pluripotency, and cellular reprogramming. Its dysregulation is linked to cancers, neurological disorders, and developmental defects. The MDB3 antibody is widely used in research to study DNA methylation dynamics, histone modification crosstalk, and NuRD complex interactions. It enables applications like chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to map MBD3 localization and function in disease models or developmental processes. Recent studies also explore its potential as a therapeutic biomarker, particularly in oncology, where aberrant DNA methylation patterns drive tumorigenesis. The antibody’s specificity and reliability make it a vital tool for dissecting epigenetic mechanisms underlying health and disease.
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