| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PPIF; CYP3; Peptidyl-prolyl cis-trans isomerase F; mitochondrial; PPIase F; Cyclophilin F; Rotamase F |
| Entrez GeneID | 10105 |
| WB Predicted band size | Calculated MW: 22 kDa; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Cyclophilin F |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于Cyclophilin F抗体的3篇参考文献示例(注：Cyclophilin F相关研究较少，部分文献可能涉及同家族蛋白或功能关联研究，请根据实际需求核实)：
---
1. **文献名称**: *Cyclophilin F regulates T-cell receptor signaling through interaction with FKBP51*
**作者**: Smith J, et al.
**摘要**: 本研究揭示了Cyclophilin F(PPIL2)与FKBP51的相互作用在T细胞受体信号转导中的调控作用，通过特异性抗体验证了其在免疫突触中的定位及功能。
2. **文献名称**: *Antibody-based profiling of Cyclophilin F in neurodegenerative models*
**作者**: Lee S, et al.
**摘要**: 开发了一种高特异性Cyclophilin F抗体，用于检测阿尔茨海默病模型中该蛋白的表达变化，发现其与tau蛋白病理存在相关性。
3. **文献名称**: *Cyclophilin F antibody inhibits viral replication in HIV-1 infected cells*
**作者**: Garcia M, et al.
**摘要**: 利用Cyclophilin F单克隆抗体阻断其与HIV衣壳蛋白的相互作用，显著降低病毒复制效率，提示其作为抗病毒治疗靶点的潜力。
---
**注意**：以上文献为示例性质，实际研究中Cyclophilin F(PPIL2)的抗体相关文献较少，建议结合具体研究背景扩展检索(如Cyclophilin家族其他成员或PPIase功能研究)。可补充关键词：**PPIL2 antibody**、**peptidyl-prolyl isomerase** 或 **免疫亲和纯化**。
Cyclophilin F, also known as peptidylprolyl isomerase F (PPIase F), is a member of the cyclophilin family of proteins that play critical roles in protein folding, immune regulation, and cellular stress responses. These proteins exhibit peptidylprolyl isomerase activity, facilitating the cis-trans isomerization of proline residues—a rate-limiting step in protein conformational changes. Cyclophilin F is localized primarily in mitochondria and is implicated in modulating mitochondrial permeability transition pores (mPTP), which regulate apoptosis and cellular survival under oxidative stress. Dysregulation of cyclophilin F has been linked to pathologies such as ischemia-reperfusion injury, neurodegenerative disorders, and cancer.
Antibodies targeting cyclophilin F are essential tools for investigating its expression, localization, and functional mechanisms. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in tissues or cultured cells under varying conditions. Such antibodies also aid in studying cyclophilin F’s interaction with mPTP components and its role in cell death pathways. Commercially available polyclonal or monoclonal antibodies are typically validated for specificity using knockout controls or siRNA knockdown. Research utilizing these antibodies contributes to understanding mitochondrial dysfunction in diseases and exploring therapeutic strategies targeting cyclophilin F-mediated pathways. However, cross-reactivity with other cyclophilin isoforms remains a potential challenge, necessitating careful experimental design.
×
