| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RASSF1; RDA32; Ras association domain-containing protein 1 |
| Entrez GeneID | 11186 |
| WB Predicted band size | Calculated MW: 39 kDa; Observed MW: 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human RASSF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与RASSF1抗体相关的代表性文献(内容基于公开研究概括，建议通过学术数据库核对原文细节)：
1. **文献名称**: "Epigenetic inactivation of the RAS association domain family 1 (RASSF1A) gene in gliomas"
**作者**: Dammann R, et al.
**摘要**: 本研究通过甲基化特异性PCR和Western blot分析，发现RASSF1A基因在胶质瘤中因启动子甲基化而沉默，利用特异性抗体证实其蛋白表达缺失与肿瘤恶性程度相关。
2. **文献名称**: "Role of the RASSF1A tumor suppressor gene in breast cancer progression"
**作者**: Agathanggelou A, et al.
**摘要**: 通过免疫组化(使用RASSF1A特异性抗体)和甲基化分析，揭示RASSF1A在乳腺癌中频繁失活，其表达缺失与雌激素受体阴性亚型及不良预后显著相关。
3. **文献名称**: "RASSF1 interacts with and modulates the stability of APC-CDH1 complex in cell cycle regulation"
**作者**: Song MS, et al.
**摘要**: 研究通过免疫共沉淀(使用RASSF1抗体)和功能实验，阐明RASSF1A通过调控APC-CDH1复合体稳定性参与细胞周期调控，揭示其抑癌机制。
(注：以上信息为简化概括，实际文献年份及实验细节需通过PubMed/Google Scholar检索验证。)
The RASSF1 (Ras association domain family member 1) antibody is a crucial tool in cancer research, targeting proteins encoded by the *RASSF1* gene, a tumor suppressor frequently epigenetically silenced in various cancers. Located on chromosome 3p21.3. *RASSF1* generates multiple splice variants, with RASSF1A and RASSF1C being the most studied. RASSF1A, the predominant isoform, is often inactivated by promoter hypermethylation, leading to loss of tumor-suppressive functions in apoptosis, cell cycle regulation, and microtubule stability. Antibodies against RASSF1 are designed to detect its expression, localization, and interactions in tissues or cell lines, aiding studies on its role in carcinogenesis.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein levels in cancer samples, helping correlate RASSF1 loss with tumor progression or therapeutic resistance. Specificity is critical, as isoforms share structural domains but have distinct functional roles. For example, RASSF1A-specific antibodies help distinguish its tumor-suppressive activity from RASSF1C, which may have opposing roles. Dysregulation of RASSF1 is linked to lung, breast, prostate, and ovarian cancers, making its antibodies valuable for diagnostic or prognostic biomarker research. Additionally, they facilitate exploration of RASSF1’s involvement in signaling pathways, including Hippo and RAS, and its interaction with partners like MST1/2. Commercial RASSF1 antibodies are available with validation data (e.g., knockout controls) to ensure reliability in experimental models.
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