| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NWASP; WASPB; N-WASP |
| Entrez GeneID | 8976 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human WASL |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇涉及N-WASP抗体的参考文献摘要(内容基于公开文献概括)：
---
1. **文献名称**: *Regulation of actin dynamics through phosphorylation of N-WASP by AMP-activated protein kinase*
**作者**: Yamaguchi H. et al.
**摘要**: 研究N-WASP在细胞骨架重组中的作用，利用特异性抗体验证AMPK磷酸化对N-WASP活性的调控，揭示其在能量应激下抑制肌动蛋白聚合的机制。
---
2. **文献名称**: *N-WASP deficiency reveals distinct pathways for cell surface and cellular protrusion*
**作者**: Snapper S.B. et al.
**摘要**: 通过N-WASP抗体进行免疫印迹和免疫荧光分析，发现N-WASP缺陷的T细胞虽能形成伪足，但无法完成稳定突触形成，证明其在细胞迁移中的关键作用。
---
3. **文献名称**: *Shigella flexneri induces membrane ruffling through N-WASP-Arp2/3 complex activation*
**作者**: Suzuki T. et al.
**摘要**: 利用抗N-WASP抗体阻断实验，证实志贺氏菌通过效应蛋白IcsA激活宿主N-WASP-Arp2/3通路，驱动细胞膜褶皱以促进细菌扩散。
---
如需具体文献来源，建议通过PubMed或Google Scholar检索标题/作者获取全文。
**Background of N-WASP Antibodies**
Neuronal Wiskott-Aldrich Syndrome Protein (N-WASP) is a key regulator of actin cytoskeleton dynamics, playing a critical role in cellular processes such as endocytosis, cell motility, and intracellular trafficking. It belongs to the WASP family of proteins and functions as a molecular switch, activating the Arp2/3 complex to nucleate actin polymerization. N-WASP activity is tightly controlled by signaling pathways involving Cdc42. PIP2. and other Rho GTPases, which induce conformational changes to expose its actin-binding VCA domain. Dysregulation of N-WASP is linked to pathologies, including cancer metastasis, immune deficiencies, and neurological disorders.
N-WASP antibodies are essential tools for studying its expression, localization, and interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate N-WASP's role in cellular mechanisms or disease contexts. Polyclonal antibodies often target multiple epitopes for enhanced detection sensitivity, while monoclonal antibodies offer high specificity for precise applications. Validated antibodies are crucial, as N-WASP shares homology with other WASP family members (e.g., WASP). Researchers also utilize phospho-specific antibodies to study post-translational modifications regulating N-WASP activity.
These antibodies have advanced understanding of N-WASP's involvement in processes like pathogen invasion (e.g., *Listeria* propulsion) and synaptic plasticity, underscoring their importance in cell biology and translational research.
×
