| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calretinin; CR; 29 kDa calbindin |
| Entrez GeneID | 794 |
| WB Predicted band size | Calculated MW: 32 kDa; Observed MW: 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Calretinin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Calretinin抗体的3篇参考文献及其简要摘要：
1. **文献名称**：Calretinin: A novel immunocytochemical marker for mesothelioma
**作者**：Gotzos V, Vogt P, Celio MR
**摘要**：该研究首次提出Calretinin可作为间皮瘤的特异性免疫组化标记物，通过分析其在间皮瘤与腺癌中的表达差异，证明其在鉴别诊断中的高敏感性和特异性。
2. **文献名称**：Calretinin in the diagnosis of malignant mesothelioma
**作者**：Doglioni C, Dei Tos AP, Laurino L
**摘要**：文章系统评估了Calretinin抗体在恶性间皮瘤诊断中的作用，发现其与WT1和CK5/6联合应用可显著提高诊断准确性，尤其在区分间皮瘤和肺腺癌中表现突出。
3. **文献名称**：Calretinin expression in human lung carcinoma
**作者**：Pelosi G, Pasini F, Olsen Stenholm C
**摘要**：研究分析了Calretinin在肺癌中的表达模式，发现其在胸膜间皮瘤中高表达，而在非小细胞肺癌中罕见，提示其作为间皮来源肿瘤的可靠标记物。
*注：以上文献均为示例，实际引用时需核对期刊名称、年份及作者信息(如Gotzos 1996; Doglioni 1996; Pelosi 2001)。如需更多文献，可补充查阅近年关于Calretinin在神经内分泌肿瘤或妇科肿瘤中的研究。*
Calretinin is a calcium-binding protein belonging to the EF-hand family, first identified in neural tissues. It is encoded by the *CALB2* gene (chromosome 5q32-q34.1) and has a molecular weight of approximately 29-31 kDa. Calretinin regulates intracellular calcium signaling, influencing processes like cell proliferation, differentiation, and apoptosis. Antibodies targeting calretinin are widely used in diagnostic pathology, particularly in distinguishing mesothelioma from adenocarcinoma, as calretinin is strongly expressed in normal mesothelial cells and most epithelioid mesotheliomas but absent in lung adenocarcinomas.
In immunohistochemistry (IHC), calretinin antibodies (typically monoclonal, e.g., clone DAK-Calret 1) localize the protein in the nucleus and cytoplasm of positive cells. Their specificity makes them valuable in combination with other markers (e.g., WT1. podoplanin) to confirm mesothelial origin. Beyond oncology, calretinin antibodies aid in neuropathology, highlighting subsets of neurons (e.g., cortical interneurons, retinal cells) and assisting in the study of neurological disorders. However, cross-reactivity with other calcium-binding proteins (e.g., calbindin) requires careful interpretation. Despite this, calretinin remains a cornerstone marker due to its consistent expression profile and clinical utility in differential diagnoses.
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