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产品描述：山羊维生素B12(VB12)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VB12含量。VB12是人体必需的维生素之一，在神经系统功能维持、DNA合成等方面发挥关键作用。研究中，其机制集中在参与细胞代谢中的甲基转移反应和催化同型半胱氨酸转化为甲硫氨酸等过程，对贫血、神经系统疾病等的防治研究有重要意义。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于科研领域中对山羊VB12代谢机制的探索，如饲料营养评估、动物生理机能研究或疾病模型中VB12动态变化的分析；组织匀浆检测功能为动物组织水平研究提供便利；为畜牧业、兽医学及基础科研领域的VB12相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：VB12
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:10 | Average % | 85 | |||||
| Range % | 80-92 | ||||||
| 1:20 | Average % | 97 | |||||
| Range % | 90-105 | ||||||
| 1:40 | Average % | 97 | |||||
| Range % | 92-104 | ||||||
| 1:80 | Average % | 92 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of goat VB12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-100 | |||||
| EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average 1000.1450.1480.147 500.2210.2270.224 250.3960.3940.395 12.50.8370.8150.826 6.251.2521.2431.248 3.122.0182.0022.010 1.562.6792.6412.660 02.7542.7162.735 | |||||||
货期：3-5 working days
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