| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FMR1; Fragile X mental retardation protein 1; FMRP; Protein FMR-1 |
| Entrez GeneID | 2332 |
| WB Predicted band size | Calculated MW: 71 kDa; Observed MW: 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human FMRP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FMRP抗体的3篇参考文献及其简要摘要：
1. **"Identification of a gene (FMR-1) containing a CGG repeat coincident with a breakpoint cluster region exhibiting length variation in fragile X syndrome"**
*Verkerk AJMH, et al. (1991)*
该研究首次克隆了与脆性X综合征相关的FMR1基因，揭示了CGG重复序列的扩增导致基因失活。研究中使用FMRP抗体确认了FMR1基因编码的蛋白在患者脑组织中的表达缺失，为后续诊断提供了分子基础。
2. **"A comparative study of FMRP antibodies for immunohistochemistry in mouse and human brain tissue"**
*Iwahashi C, et al. (2009)*
作者系统比较了多种市售FMRP抗体的特异性与敏感性，发现某些抗体在小鼠和人类脑组织中的交叉反应性差异显著。研究强调了抗体选择对实验结果可靠性的重要性，并推荐了适用于不同实验场景的抗体。
3. **"FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism"**
*Darnell JC, et al. (2011)*
通过免疫沉淀结合高通量测序，研究揭示了FMRP通过结合靶mRNA调控核糖体移动的分子机制。实验中利用特异性FMRP抗体富集结合复合物，证实其在突触蛋白翻译抑制中的关键作用，为脆性X综合征的病理机制提供了新见解。
*注：以上文献均聚焦于FMRP抗体的应用，涵盖基础机制、实验工具验证及疾病关联研究。若需扩展，可补充FMRP表达模式研究(如Tamanini F, et al. 1997)等相关文献。*
FMRP (Fragile X Mental Retardation Protein) is an RNA-binding protein encoded by the *FMR1* gene, crucial for regulating synaptic plasticity and protein synthesis in neuronal development. Mutations in *FMR1*, particularly CGG trinucleotide repeat expansions, lead to Fragile X syndrome (FXS), a leading genetic cause of intellectual disability and autism spectrum disorders. FMRP antibodies are essential tools in neuroscience and biomedical research, enabling the detection and quantification of FMRP expression in tissues and cellular models. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study FMRP's localization, interaction partners, and functional deficits in FXS.
Commercially available FMRP antibodies are typically raised against specific epitopes of the protein, though variability in specificity across vendors necessitates careful validation. Recent studies also explore FMRP's role beyond neurons, including its expression in glial cells and non-neural tissues. Challenges persist in standardizing antibody-based assays due to FMRP's multiple isoforms and tissue-specific expression patterns. Additionally, FMRP antibodies contribute to diagnostic workflows, aiding in confirming FXS cases with atypical genetic presentations. Emerging technologies, such as CRISPR-engineered models and single-cell sequencing, increasingly rely on validated FMRP antibodies to dissect the protein’s role in neurodevelopmental pathways and potential therapeutic targets. Despite advancements, cross-reactivity and batch variability remain critical considerations in experimental design.
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