| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ROCK1; Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated; coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK |
| Entrez GeneID | 6093 |
| WB Predicted band size | Calculated MW: 158 kDa; Observed MW: 158 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ROCK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"ROCK1 regulates apoptosis via interaction with PP2Ac"** by Nakagawa et al.
*摘要：研究通过ROCK1抗体验证其在细胞凋亡中的作用，发现ROCK1与蛋白磷酸酶PP2Ac的相互作用调控线粒体膜通透性，促进凋亡信号通路。*
2. **"ROCK1 promotes axonal growth in neuronal development"** by Shi et al.
*摘要：利用ROCK1特异性抗体进行免疫印迹和免疫荧光，揭示其在神经元轴突生长中的关键功能，并探讨其抑制剂对神经损伤修复的潜在价值。*
3. **"ROCK1 mediates cardiac fibrosis through TGF-β signaling"** by Zhou et al.
*摘要：通过抗体阻断实验证实ROCK1在心脏纤维化中的核心作用，发现其通过激活TGF-β/Smad通路促进成纤维细胞活化，为心血管疾病治疗提供新靶点。*
4. **"Selective ROCK1 inhibition suppresses tumor metastasis"** by Patel et al.
*摘要：研究采用ROCK1抗体筛选小分子抑制剂，证明特异性抑制ROCK1(而非ROCK2)可显著降低乳腺癌细胞侵袭和转移能力，提示其作为抗癌靶点的潜力。*
The ROCK1 (Rho-associated coiled-coil containing protein kinase 1) antibody is a critical tool for studying the role of ROCK1. a serine/threonine kinase involved in regulating cytoskeletal dynamics, cell adhesion, migration, and apoptosis. ROCK1 is activated by Rho GTPases (e.g., RhoA) and functions as a key mediator of the Rho-ROCK signaling pathway, influencing processes such as stress fiber formation, smooth muscle contraction, and neurite retraction. Dysregulation of ROCK1 has been linked to cardiovascular diseases (e.g., hypertension, atherosclerosis), cancer metastasis, neurological disorders, and fibrosis.
ROCK1 antibodies are designed to detect endogenous ROCK1 protein (~160 kDa) in applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specificity is crucial due to structural homology with ROCK2. a closely related isoform. High-quality antibodies target unique epitopes in ROCK1’s kinase domain or C-terminal region to minimize cross-reactivity. Researchers use these antibodies to explore ROCK1 expression patterns, subcellular localization, and activation status (e.g., phosphorylated forms) in disease models or therapeutic contexts, such as evaluating ROCK inhibitors. Validation via knockout controls or siRNA knockdown ensures reliability. The development of ROCK1-targeted therapies underscores the antibody’s importance in both basic research and drug discovery.
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