| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X |
| Entrez GeneID | 3014 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H2A.X |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于Histone H2A.X抗体的经典参考文献，涵盖其关键发现和应用：
---
1. **"DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139"**
- **作者**: Rogakou, E.P., Pilch, D.R., Orr, A.H., Ivanova, V.S., Bonner, W.M.
- **摘要**: 该研究首次报道了DNA双链断裂(DSBs)诱导H2A.X在丝氨酸139位点的磷酸化(γ-H2A.X)，并开发了特异性抗体用于检测。通过免疫荧光技术，揭示了γ-H2A.X病灶的形成与DNA损伤修复焦点共定位，成为DSBs的标志性分子事件。
2. **"Characteristics of γ-H2AX foci at DNA double-strand break sites"**
- **作者**: Pilch, D.R., Sedelnikova, O.A., Redon, C., Celeste, A., Nussenzweig, A., Bonner, W.M.
- **摘要**: 本文系统评估了不同DNA损伤源(如电离辐射、化疗药物)诱导的γ-H2A.X焦点特征，利用特异性抗体验证了其与DSBs的定量关系，并证实焦点数量与损伤程度呈正相关，为后续生物标志物研究奠定基础。
3. **"Quantitative detection of 125IdU-induced DNA double-strand breaks with γ-H2AX antibody"**
- **作者**: Lu, C., Zhu, F., Cho, Y.Y., Tang, F., et al.
- **摘要**: 研究通过流式细胞术结合γ-H2A.X抗体，定量分析了放射性同位素(125IdU)诱导的DSBs，验证了抗体在低剂量辐射检测中的灵敏度，为辐射暴露的生物剂量测定提供了可靠方法。
---
这些文献涵盖了H2A.X抗体的开发、机制研究及实际应用，适用于DNA损伤检测、修复研究及实验方法学参考。
Histone H2A.X is a variant of the H2A histone family, playing a critical role in maintaining genomic stability and coordinating DNA damage response (DDR). Its phosphorylation at serine 139 (γH2A.X) is a hallmark of DNA double-strand breaks (DSBs), serving as an early signaling event to recruit repair proteins. Antibodies targeting H2A.X, particularly phospho-specific γH2A.X antibodies, are essential tools in molecular biology and cancer research for detecting and quantifying DNA damage.
These antibodies enable visualization of DSB foci via immunofluorescence, immunoblotting, or flow cytometry, providing insights into cellular responses to genotoxic agents, radiation, or replication stress. γH2A.X foci are widely used as biomarkers for DNA damage in studies of cancer therapy efficacy, aging, and genomic instability disorders. Additionally, they help elucidate DDR pathways involving kinases like ATM, ATR, and DNA-PK, which phosphorylate H2A.X in response to damage.
Researchers must validate H2A.X antibodies for species specificity, phosphorylation sensitivity, and cross-reactivity, as variations exist across experimental models. Non-phosphorylated H2A.X antibodies are also employed to assess total protein levels or chromatin dynamics. Together, these tools have advanced our understanding of DNA repair mechanisms, drug toxicity screening, and the role of epigenetic regulation in disease.
×
