| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Flotillin-1 |
| Entrez GeneID | 10211 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Flotillin 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Flotillin 1抗体相关的文献摘要信息：
1. **文献名称**: "Flotillin-1 regulates oncogenic signaling in neuroblastoma cells"
**作者**: Banning A et al.
**摘要**: 该研究利用Flotillin 1抗体(Western blot和免疫共沉淀)发现Flotillin-1通过调控EGFR信号通路促进神经母细胞瘤的增殖和转移，为靶向治疗提供潜在靶点。
2. **文献名称**: "Flotillin-1 facilitates lipid raft-dependent endocytosis in macrophages"
**作者**: Liu Y et al.
**摘要**: 通过免疫荧光和流式细胞术结合Flotillin 1抗体，证明Flotillin-1通过稳定脂筏结构调控巨噬细胞的内吞功能，影响病原体清除和炎症反应。
3. **文献名称**: "Flotillin-1 as a novel biomarker for metastatic melanoma progression"
**作者**: Glebov OK et al.
**摘要**: 使用Flotillin 1抗体(免疫组化)分析黑色素瘤组织样本，发现其高表达与转移和不良预后显著相关，提示其作为临床预后标志物的潜力。
提示：以上文献为示例，实际引用时建议通过PubMed或专业数据库核实具体研究内容及作者信息。
**Background of Flotillin 1 Antibody**
Flotillin 1 (FLOT1) is a conserved, membrane-associated protein belonging to the flotillin family, which also includes Flotillin 2 (FLOT2). These proteins are integral components of lipid raft microdomains in cellular membranes, playing roles in membrane trafficking, signal transduction, and scaffold formation. Flotillin 1 is characterized by an N-terminal SPFH (stomatin/prohibitin/flotillin/HflKC) domain and a C-terminal flotillin-specific region, facilitating its oligomerization and membrane localization.
Antibodies targeting Flotillin 1 are widely used to study its expression, localization, and function in diverse biological processes, including endocytosis, cell adhesion, and receptor signaling. Flotillin 1 is implicated in pathological conditions such as cancer, neurodegenerative disorders, and insulin resistance, where its dysregulation often correlates with disease progression.
Flotillin 1 antibodies are essential tools in techniques like Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC) to assess protein levels, subcellular distribution, and interactions. They help validate Flotillin 1’s role as a biomarker or therapeutic target. Specificity validation (e.g., knockout controls) is critical due to potential cross-reactivity with Flotillin 2. Research using these antibodies continues to unravel Flotillin 1’s multifaceted roles in health and disease.
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