| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | XBP1; TREB5; XBP2; X-box-binding protein 1; XBP-1; Tax-responsive element-binding protein 5 |
| Entrez GeneID | 7494 |
| WB Predicted band size | Calculated MW: 29 kDa; Observed MW: 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Recombinant protein of human XBP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于XBP1抗体的参考文献示例，包含文献名称、作者及摘要内容概括：
1. **"XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor"**
- **作者**: Yoshida, H., et al. (2001)
- **摘要**: 研究揭示了XBP1通过内质网应激(ER stress)激活的分子机制，利用特异性抗体检测XBP1的剪接形式(spliced XBP1)，证实其在未折叠蛋白反应(UPR)中的转录调控作用。
2. **"Plasma cell differentiation requires the transcription factor XBP-1"**
- **作者**: Lee, A.H., et al. (2006)
- **摘要**: 通过Western blot和免疫荧光技术，使用XBP1抗体证明XBP1在浆细胞分化中的必要性，发现XBP1缺失导致抗体分泌功能受损。
3. **"A transgenic mouse model for monitoring endoplasmic reticulum stress"**
- **作者**: Iwawaki, T., et al. (2008)
- **摘要**: 开发了一种基于XBP1剪接活性的荧光报告系统，研究中通过XBP1抗体验证剪接效率，为内质网应激的体内检测提供工具。
4. **"XBP1 promotes triple-negative breast cancer by controlling the HIF1α pathway"**
- **作者**: Chen, X., et al. (2014)
- **摘要**: 利用XBP1抗体进行免疫组化分析，发现XBP1在三阴性乳腺癌中高表达，并通过调控HIF1α通路促进肿瘤生长和转移。
**注意**：以上文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar核对完整信息及最新研究。
The X-box binding protein 1 (XBP1) antibody is a critical tool for studying the unfolded protein response (UPR), a cellular stress pathway activated by endoplasmic reticulum (ER) stress. XBP1 is a transcription factor encoded by the *XBP1* gene, existing in two isoforms: the unspliced (XBP1u) and spliced (XBP1s) forms. Under ER stress, the endoribonuclease IRE1α cleaves XBP1u mRNA, removing a 26-nucleotide intron to generate XBP1s, which translocates to the nucleus and activates UPR target genes involved in protein folding, degradation, and ER biogenesis.
XBP1 antibodies are widely used in research to detect and differentiate between XBP1u (inactive) and XBP1s (active) isoforms, aiding in the study of ER stress in diseases such as cancer, neurodegenerative disorders (e.g., Alzheimer’s), diabetes, and inflammatory conditions. These antibodies are essential for techniques like Western blotting, immunofluorescence, and immunohistochemistry, enabling researchers to assess UPR activation in cellular or tissue models. Commercially available XBP1 antibodies often target specific regions, such as the C-terminal domain unique to XBP1s or shared epitopes between isoforms. Validation is critical, as cross-reactivity with other proteins or failure to distinguish isoforms can lead to misinterpretation. Research involving XBP1 antibodies has advanced understanding of cellular stress mechanisms and therapeutic targeting of UPR-related pathologies.
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