首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人中部前心钠肽(MR-proANP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MR-proANP含量。试剂盒检测范围为39 pmol/L-2500 pmol/L，该产品适用于心血管疾病机制研究、动物模型中心功能评估、药物干预效果监测等科研领域，为探索心力衰竭、心肌缺血等疾病的分子机制提供定量分析工具。试剂盒操作流程标准化，配套完整实验组分，支持实验室开展高通量样本检测，满足基础医学研究和生物标志物开发需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MR-proANP
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：39 pmol/L-2500 pmol/L
灵敏度：9.8 pmol/L
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MR-proANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 94-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 83-94 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of human MR-proANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 102 | 92-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/LOD1OD2AverageCorrected 25002.1551.9882.0721.926 12501.6971.6271.6621.516 6251.1601.0881.1240.978 3120.6920.7080.7000.554 1560.4140.4250.4200.274 780.2830.2770.2800.134 390.1970.1910.1940.048 00.1480.1440.146 | ||||||
货期：3-5 working days
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