WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | MtTF1; mtTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; Transcription factor 6 |
Entrez GeneID | 7019 |
WB Predicted band size | Calculated MW: 29 kDa; Observed MW: 24 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | A synthetic peptide of human mtTFA |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及mtTFA(线粒体转录因子A)抗体的代表性文献示例(注:内容为示例性质,实际引用时请核对原文):
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1. **文献名称**: *"Mitochondrial transcription factor A regulates mitochondrial DNA maintenance and determines cell sensitivity to oxidative stress"*
**作者**: Larsson NG, et al.
**摘要**: 本研究利用mtTFA抗体通过Western blot和免疫荧光技术,分析小鼠模型中mtTFA蛋白的表达水平与线粒体DNA拷贝数的关系,发现mtTFA缺乏导致线粒体功能障碍并增加细胞对氧化应激的敏感性。
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2. **文献名称**: *"TFAM coordinates transcriptional changes in mitochondrial biogenesis and neurodegeneration"*
**作者**: Hayashi Y, et al.
**摘要**: 通过免疫组化结合mtTFA抗体,研究者在阿尔茨海默病模型中发现mtTFA在神经元线粒体中的定位异常,表明其与神经退行性疾病中线粒体能量代谢紊乱密切相关。
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3. **文献名称**: *"Cardiac-specific deletion of mitochondrial transcription factor A promotes mitochondrial dysfunction in ischemia/reperfusion injury"*
**作者**: Ikeuchi M, et al.
**摘要**: 使用mtTFA抗体检测心肌细胞中线粒体TFAM的分布,揭示其在心肌缺血再灌注损伤中通过调控线粒体基因表达影响细胞存活,为心脏保护机制提供新见解。
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**备注**:以上文献标题及内容为示例,实际文献可能需要通过PubMed、Google Scholar等平台检索关键词(如“mtTFA antibody”“TFAM immunohistochemistry”)获取。建议优先选择近5年高引用研究以确保时效性。
**Background of mtTFA Antibody**
Mitochondrial transcription factor A (mtTFA), also known as TFAM, is a nuclear-encoded protein critical for mitochondrial DNA (mtDNA) replication, transcription, and maintenance. It binds to mtDNA, compacting it into nucleoid structures and regulating its stability and accessibility. mtTFA contains two high-mobility group (HMG)-box domains that facilitate DNA binding and bending, essential for mtDNA packaging and replication.
mtTFA is indispensable for mitochondrial biogenesis and cellular energy production, as mtDNA encodes components of the electron transport chain (ETC) required for ATP synthesis. Dysregulation of mtTFA is linked to mitochondrial disorders, neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s), cancer, and metabolic syndromes. Reduced mtTFA levels correlate with mtDNA depletion, impaired oxidative phosphorylation, and increased oxidative stress.
mtTFA antibodies are vital tools for studying mitochondrial function and disease mechanisms. They enable detection of mtTFA expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers use these antibodies to investigate mtTFA’s role in mitochondrial dynamics, apoptosis, and its interaction with other proteins (e.g., POLG, mitochondrial RNA polymerase). Commercial mtTFA antibodies are typically validated for specificity across human, mouse, and rat models.
Understanding mtTFA’s regulation and interactions through antibody-based assays provides insights into therapeutic strategies targeting mitochondrial dysfunction. Such studies are crucial for advancing treatments for diseases linked to mtDNA instability and energy metabolism defects.
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