| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DOC2; DOC-2 |
| Entrez GeneID | 1601 |
| WB Predicted band size | Calculated MW: 82 kDa; Observed MW: 96 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human DAB2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于DAB2抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Disabled-2 modulates TGF-β signaling in mammary gland epithelial cells"*
**作者**：Hocevar, B.A., et al.
**摘要**：该研究探讨了DAB2蛋白在乳腺上皮细胞中对TGF-β信号通路的调控作用。通过Western blot和免疫共沉淀实验，作者发现DAB2抗体能特异性识别内源性DAB2蛋白，并揭示其通过结合Smad蛋白抑制TGF-β介导的细胞增殖抑制和凋亡。
2. **文献名称**：*"Epigenetic silencing of the disabled-2 (DAB2) gene in human ovarian carcinomas"*
**作者**：Mok, S.C., et al.
**摘要**：该研究分析了DAB2在卵巢癌中的表达缺失机制，发现其启动子甲基化导致表达下调。通过免疫组化(使用DAB2特异性抗体)和甲基化特异性PCR，证实DAB2作为肿瘤抑制因子在卵巢癌发生中的作用。
3. **文献名称**：*"DAB2 suppresses mTOR activity to regulate macrophage polarization and inflammatory responses"*
**作者**：Cheong, H., et al.
**摘要**：研究揭示了DAB2在巨噬细胞极化中的功能。利用DAB2抗体进行免疫荧光和流式细胞术，发现DAB2通过抑制mTOR通路促进M2型抗炎巨噬细胞分化，从而调控炎症反应和组织修复。
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以上文献均涉及DAB2抗体的实验应用(如蛋白检测、定位分析)，并围绕DAB2在癌症、免疫或信号通路中的功能展开。如需具体文献年份或期刊，可进一步补充数据库检索。
The Disabled-2 (DAB2) protein is a adaptor molecule involved in cellular signaling and trafficking, primarily known for its role in regulating transforming growth factor-beta (TGF-β) and Wnt signaling pathways. It acts as a tumor suppressor by modulating cell proliferation, differentiation, and apoptosis. DAB2 is widely expressed in epithelial tissues and immune cells, where it facilitates clathrin-mediated endocytosis and maintains cell polarity. Its loss or downregulation is associated with cancer progression, metastasis, and drug resistance in various malignancies, including ovarian, breast, and prostate cancers.
DAB2 antibodies are essential tools for detecting and studying this protein in research applications. They are commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess DAB2 expression levels and localization in tissues or cell lines. These antibodies help elucidate DAB2's interaction with signaling molecules (e.g., SMAD proteins, β-catenin) and its involvement in pathways linked to carcinogenesis, fibrosis, and immune regulation. Some antibodies specifically target distinct DAB2 isoforms (p96 and p67) arising from alternative splicing. Commercial DAB2 antibodies are typically raised against epitopes in the N-terminal region, which is critical for its PTB (phosphotyrosine-binding) domain function. Validation includes testing in knockout models or siRNA-treated cells to confirm specificity, given its structural similarity to other adaptor proteins.
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