| WB | 1/500-1/1000 | Human,Rat,Hamster |
| IF | 1/20 | Human,Rat,Hamster |
| IHC | 1/50-1/100 | Human,Rat,Hamster |
| ICC | 1/50-1/200 | Human,Rat,Hamster |
| FCM | 咨询技术 | Human,Rat,Hamster |
| Elisa | 咨询技术 | Human,Rat,Hamster |
| Aliases | CDK2; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase |
| Entrez GeneID | 1017 |
| WB Predicted band size | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat,Hamster |
| Immunogen | A synthetic peptide of human Cdk2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于CDK2抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*"Cell cycle kinases in cancer: from biology to therapy"*
**作者**：Malumbres, M.
**摘要**：该综述讨论了细胞周期依赖性激酶(如CDK2)在肿瘤发生中的作用，重点介绍了CDK2在调控G1/S期转换中的功能，并提及了通过特异性抗体检测CDK2表达及活性在癌症研究中的应用，为靶向治疗提供依据。
2. **文献名称**：*"Ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligase regulates cell cycle progression"*
**作者**：Pagano, M. 等
**摘要**：本文研究了Cyclin E的泛素化降解机制，利用CDK2抗体进行免疫共沉淀实验，揭示了CDK2与Cyclin E复合物的动态调控，以及其在细胞周期失调相关癌症中的潜在意义。
3. **文献名称**：*"Selective CDK2 inhibition through targeting the allosteric regulatory site"*
**作者**：Tadesse, S. 等
**摘要**：该研究开发了一种选择性CDK2小分子抑制剂，通过CDK2抗体进行Western blot和流式细胞术分析，验证了抑制剂对CDK2活性的特异性抑制及其在抗肿瘤治疗中的效果。
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以上文献涵盖了CDK2抗体的应用场景，包括基础机制研究(如蛋白相互作用)、肿瘤靶向治疗开发及细胞周期调控分析，为相关领域提供参考。
CDK2 (Cyclin-Dependent Kinase 2) is a serine/threonine kinase critical for regulating cell cycle progression, primarily during the G1/S phase transition and S phase entry. It partners with cyclins E and A to phosphorylate key substrates like retinoblastoma (Rb) protein, promoting DNA replication and cell division. CDK2 activity is tightly controlled by inhibitory phosphorylation (e.g., p21. p27) and cyclin binding. Dysregulation of CDK2 is implicated in cancers, as overexpression or hyperactivation can drive uncontrolled proliferation, metastasis, and therapy resistance in tumors such as breast, ovarian, and lung cancers.
CDK2 antibodies are essential tools for studying its expression, localization, and activation in cellular models and tissues. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specific antibodies targeting phosphorylated CDK2 (e.g., Thr160) help assess its active state, while pan-CDK2 antibodies detect total protein levels. Validation often includes testing in CDK2-knockout cell lines to confirm specificity.
Research applications include exploring CDK2's role in cancer biology, identifying therapeutic targets, and evaluating CDK inhibitors (e.g., dinaciclib). Recent interest also focuses on CDK2's non-canonical functions in meiosis, metabolism, and DNA repair. Reliable CDK2 antibodies underpin mechanistic studies and translational efforts to develop precision oncology therapies.
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