首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠干扰素α2(IFNA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IFNA2含量。IFNA2 即干扰素α2，是一种细胞因子。它具有抗病毒、抗肿瘤和免疫调节等作用。其研究机制主要在于它能与细胞表面受体结合，激活信号通路，诱导下游多种抗病毒和免疫相关基因表达，从而发挥生物学效应。试剂盒检测范围为9.38 pg/mL-600 pg/mL，适用于体外实验中IFNA2表达水平的动态监测，例如病毒学研究中宿主免疫应答评估、免疫调节机制探索或炎症相关疾病模型构建等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ifna2Interferon alpha-2 ELISA Kit; IFN-alpha-2 ELISA Kit
缩写：IFNA2
Uniprot No.：P01573
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：9.38 pg/mL-600 pg/mL
灵敏度：2.35 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 98-107 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 95-99 | ||||||
回收率：
| The recovery of mouse IFNA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 89-98 | |||||
| EDTA plasma (n=4) | 98 | 94-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected6002.6512.5362.5942.4403002.0491.9281.9891.8351501.3761.3151.3461.192750.8920.9520.9220.76837.50.5680.5290.5490.39518.750.3740.3640.3690.2159.40.2370.2410.2390.08500.1500.1570.154 | |||||||
货期：3-5 working days
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