产品描述：大鼠成纤维细胞生长因子21(FGF21)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF21含量。FGF21是一种成纤维细胞生长因子。它在糖脂代谢等生理过程中发挥重要作用，具有改善胰岛素敏感性、降低血糖和血脂的潜在功效。研究机制主要围绕其与受体结合，激活下游信号通路，调节细胞代谢活动，为代谢性疾病治疗带来新方向。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品适用于代谢性疾病基础研究领域，包括肥胖、糖尿病模型中的FGF21动态变化分析，或肝脏代谢调控机制的探索。为研究者开展能量代谢相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：FGF21
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:4 | Average % | 106 | ||||
| Range % | 102-114 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat FGF21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-90 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.6462.7722.7092.5907501.9731.9321.9531.8343751.2171.3021.2601.141187.50.7890.7930.7910.672940.4630.4490.4560.337470.3020.2810.2920.17323.50.1950.2050.2000.08100.1210.1170.119 | ||||||
货期：3-5 working days
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