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Rabbit Monoclonal Raf1 Antibody

  • 中文名: Raf1抗体
  • 别    名: RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
货号: IPDX22662
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Entrez GeneID5894
WB Predicted band sizeCalculated MW: 73 kDa; Observed MW: 73 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human Raf1
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3-4条关于Raf1抗体的参考文献示例(内容为虚构,仅供格式参考):

1. **文献名称**: "Structural analysis of Raf1 kinase and its interaction with antibodies"

**作者**: Smith A, et al.

**摘要**: 通过X射线晶体学解析Raf1激酶结构,并验证特异性抗体对Raf1活性位点的靶向结合能力,为癌症治疗中的Raf1抑制策略提供依据。

2. **文献名称**: "Raf1 signaling in cancer: Antibody-based detection and therapeutic implications"

**作者**: Lee B, et al.

**摘要**: 开发高特异性Raf1抗体用于肿瘤组织检测,发现Raf1过表达与结直肠癌患者预后不良相关,并探索抗体偶联药物在临床前模型中的疗效。

3. **文献名称**: "Dynamic regulation of Raf1 phosphorylation revealed by phospho-specific antibodies"

**作者**: Chen X, et al.

**摘要**: 设计磷酸化位点特异性Raf1抗体,揭示Ras激活后Raf1在Ser338/Tyr341位点的磷酸化动态变化及其对MAPK信号通路的调控机制。

4. **文献名称**: "Monoclonal antibody generation for Raf1 functional studies"

**作者**: Tanaka K, et al.

**摘要**: 报道一种新型抗Raf1单克隆抗体的开发流程,验证其在免疫沉淀、Western blot及免疫荧光中的应用,并成功用于斑马鱼模型中的Raf1蛋白定位研究。

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**注**:以上文献为示例性内容,实际文献需通过PubMed、Web of Science等数据库检索。建议使用关键词如"Raf1 antibody application"或"Raf1 kinase detection"查找近5年研究以获取最新进展。

背景信息

The Raf1 antibody is a crucial tool in studying the Raf1 protein (also known as c-Raf or CRAF), a serine/threonine kinase central to the MAPK/ERK signaling pathway. Raf1 plays a pivotal role in regulating cell proliferation, differentiation, and survival by transmitting extracellular signals (e.g., growth factors) to downstream effectors like MEK and ERK. Dysregulation of Raf1 is implicated in cancers, metabolic disorders, and developmental abnormalities, making it a key target in biomedical research.

Raf1 antibodies are designed to detect and quantify the protein in various experimental settings, including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. They are available as monoclonal or polyclonal antibodies, often generated in hosts like rabbits or mice using immunogenic peptide sequences specific to Raf1. These antibodies typically target distinct regions of the protein, such as the N-terminal regulatory domain or the C-terminal kinase domain, enabling researchers to study its activation state (e.g., phosphorylated vs. unphosphorylated forms) or interactions with binding partners like Ras or 14-3-3 proteins.

Validation of Raf1 antibodies is critical, as off-target binding can occur due to homology with other Raf isoforms (A-Raf, B-Raf). Applications span basic research (e.g., signaling pathway mapping) and clinical studies (e.g., assessing Raf1 overexpression in tumors). Commercial antibodies from suppliers like Cell Signaling Technology, Abcam, and Santa Cruz Biotechnology are widely used, often accompanied by protocols and validation data (e.g., knockout cell line controls). Recent advancements include phospho-specific antibodies to study Raf1 activation dynamics in response to targeted therapies.

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