首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠过氧化物酶体增殖激活物受体γ辅激活因子1α(PPARGC1A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PPARGC1A含量。PPARGC1A是重要的转录共激活因子，在能量代谢、线粒体生物合成等生理过程中起关键作用。研究机制上，它可与多种转录因子相互作用，调节相关基因表达以响应环境变化，其功能异常与代谢疾病、神经退行性疾病等密切相关。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于探索小鼠模型中PPARGC1A在能量代谢调控、糖尿病/肥胖病理机制、运动适应性及线粒体功能相关研究中的动态变化。支持科研人员高效开展细胞能量代谢研究、疾病模型评估及分子调控网络解析等基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ppargc1a ELISA Kit; Pgc1 ELISA Kit; Pgc1a ELISA Kit; Ppargc1 ELISA Kit; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; PGC-1-alpha ELISA Kit; PPAR-gamma coactivator 1-alpha ELISA Kit; PPARGC-1-alpha ELISA Kit
缩写：PPARGC1A
Uniprot No.：O70343
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PPARGC1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 97-108 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of mouse PPARGC1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-97 | ||||
| EDTA plasma (n=4) | 99 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.7302.5822.6562.4667502.1812.0762.1291.9393751.3651.3451.3551.165187.50.8710.8320.8520.662940.5500.5410.5460.356470.3850.3780.3820.19223.50.2880.2790.2840.09400.1920.1880.190 | ||||||
货期：3-5 working days
×
