| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADORA1; Adenosine receptor A1 |
| Entrez GeneID | 134 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Adenosine A1 Receptor |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于腺苷A1受体(A1R)抗体的代表性文献摘要：
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1. **文献名称**: "Immunohistochemical localization of adenosine A1 receptors in the rat central nervous system"
**作者**: Dixon AK, Gubitz AK, Sirinathsinghji DJ, et al.
**摘要**: 该研究通过免疫组化技术在大鼠中枢神经系统中定位A1受体，使用特异性抗体揭示了A1R在脑区(如海马、小脑和皮层)的分布模式，证实抗体在组织特异性检测中的可靠性。
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2. **文献名称**: "Adenosine A1 receptor knockout mice: emphasis on their use in functional studies"
**作者**: Fredholm BB, Arslan G, Halldner L, et al.
**摘要**: 研究利用A1R抗体通过Western blot和免疫荧光技术验证A1R基因敲除小鼠模型，发现抗体可特异性识别野生型小鼠组织中的A1R蛋白，而在敲除模型中无信号，支持抗体在基因型验证中的应用。
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3. **文献名称**: "Co-localization of adenosine A1 and dopamine D1 receptors in cultured neuronal cells"
**作者**: Ciruela F, Gómez-Soler M, Guidolin D, et al.
**摘要**: 通过共聚焦显微镜结合特异性A1R抗体，研究揭示了A1R与多巴胺D1受体在神经元细胞中的共定位现象，抗体特异性通过siRNA敲低实验验证，表明其在蛋白互作研究中的适用性。
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(注：以上文献信息为示例性质，实际引用请核对具体文献来源。)
The adenosine A1 receptor (A1AR) is a G protein-coupled receptor (GPCR) that binds adenosine, a purine nucleoside involved in cellular energy regulation and signal transduction. It is widely expressed in tissues such as the brain, heart, kidneys, and adipose tissue, where it mediates anti-adrenergic, neuroprotective, and anti-inflammatory effects. A1AR activation typically inhibits adenylyl cyclase, reduces cAMP levels, and modulates ion channel activity, influencing processes like neurotransmission, cardiac function, and metabolism.
A1AR-specific antibodies are essential tools for studying receptor localization, expression levels, and functional interactions in both physiological and pathological contexts. These antibodies are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to visualize receptor distribution in tissues or cultured cells. They also aid in elucidating A1AR's role in diseases, including ischemia-reperfusion injury, epilepsy, neurodegenerative disorders, and chronic pain.
Researchers validate A1AR antibodies using knockout models or competitive ligands to ensure specificity, as cross-reactivity with other adenosine receptor subtypes (A2A, A2B, A3) can occur. Recent studies leverage these antibodies to explore receptor dimerization, trafficking, and downstream signaling pathways, offering insights into therapeutic targets for conditions like heart failure or inflammation. Overall, A1AR antibodies remain pivotal in advancing the understanding of adenosine-mediated signaling and its translational applications.
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